Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-ChipVarone Antonio 1, Adya Panchal 2, Magdalena Kasendra 3, Barrile Riccardo 2,3
1Merk Sharp & Dohme LLC, 2Department of Biomedical Engineering, University of Cincinnati, 3Center for Stem Cell and Organoid Medicine, Cincinnati Children's Hospital Medical Center
The present protocol describes the capabilities and the essential culture modalities of the Open-Top Organ-Chip for the successful establishment and maturation of full-thickness organ-on-chip cultures of primary tissues (skin, alveolus, airway, and intestine), providing the opportunity to investigate different functional aspects of the human epithelial/mesenchymal and vascular niche interface in vitro.
