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Affiliated Hospital of Xuzhou Medical University

3 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling

Hong Yang¹, Shan Yu Fung², Aihua Bao¹, Qiang Li¹, Stuart E. Turvey²

¹Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, ²Department of Pediatrics, BC Children's Hospital and University of British Columbia

Toll-like receptor (TLR) signaling plays an important role in the pathophysiology of many human inflammatory diseases, and regulating TLR responses by bioactive nanoparticles is anticipated to be beneficial in many inflammatory conditions. THP-1 cell-based reporter cells provide a versatile and robust screening platform for identifying novel inhibitors of TLR signaling.

Cancer Research

Ex Vivo Organoid Model of Adenovirus-Cre Mediated Gene Deletions in Mouse Urothelial Cells

Dongbo Xu¹, Li Wang¹, Kyle Wieczorek¹, Yanqing Wang², Xiaojing Zhang², David W. Goodrich², Qiang Li^1,2

¹Department of Urology, Roswell Park Comprehensive Cancer Center, ²Department of Pharmacology and Therapeutics, Roswell Park Comprehensive Cancer Center

This protocol describes the process of the generation and characterization of mouse urothelial organoids harboring deletions in genes of interest. The methods include harvesting mouse urothelial cells, ex vivo transduction with adenovirus driving Cre expression with a CMV promoter, and in vitro as well as in vivo characterization.

Bioengineering

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

Jingyu Zhang^*1,2, Bingkun Zhao^*1, Wuhan Wei^*1,2, Dan Wang^3,4,5, Haoyu Wang^1,2, Aijun Zhang¹, Changbo Tao¹, Xueyang Li¹, Qiang Li¹, Peisheng Jin¹

¹Department of Plastic Surgery, Affiliated Hospital of Xuzhou Medical University, ²Graduate School, Xuzhou Medical University, ³Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong, ⁴School of Biomedical Sciences, The Chinese University of Hong Kong, ⁵Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park

The present protocol describes a prospective, randomized, controlled clinical study that evaluates a human umbilical cord mesenchymal stem cell injection for the treatment of chronic diabetic foot ulcers.