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University of Kent

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster
Hrvoje Augustin 1, Marcus J. Allen 2, Linda Partridge 1
1Institute of Healthy Ageing, and GEE, University College London - UCL, 2School of Biosciences, University of Kent

The Giant Fiber System is a simple neuronal circuit of adult Drosophila melanogaster containing the largest neurons in the fly. We describe the protocol for monitoring synaptic transmission through this pathway by recording post synaptic potentials in dorsal longitudinal (DLM) and tergotrochanteral (TTM) muscles following direct stimulation of the Giant Fiber interneurons.

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Behavior

Holistic Facial Composite Creation and Subsequent Video Line-up Eyewitness Identification Paradigm
Josh P. Davis 1, Andreea C. Maigut 1, Darrick Jolliffe 2, Stuart J. Gibson 3, Chris J. Solomon 3
1Department of Psychology, Social Work and Counselling, University of Greenwich, 2Department of Criminology, University of Greenwich, 3School of Physical Sciences, University of Kent

This applied experimental paradigm replicates circumstances by which an eyewitness to a real crime may create a holistic facial composite of the culprit from memory, and then attempt to identify the culprit from a video line-up containing the culprit or one in which he or she is not present.

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Bioengineering

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
Ming Toh 1,2,3,4, Kameshwari Chengan 5, Tanith Hanson 5, Paul S. Freemont 1,2,3,4,6,7, Simon J. Moore 5
1Centre for Synthetic Biology and Innovation, South Kensington Campus, 2Department of Medicine, South Kensington Campus, 3Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, 4Sir Alexander Fleming Building, South Kensington Campus, 5School of Biosciences, Division of Natural Sciences, University of Kent, 6UK Dementia Research Institute Care Research and Technology Centre, Imperial College London; Hammersmith Campus, 7UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry, Imperial College Translation & Innovation Hub

This protocol details an enhanced method for synthesizing high yields of recombinant proteins from a Streptomyces venezuelae cell-free transcription-translation (TX-TL) system.

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Biology

Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from E. coli
Bree R. Streather 1, Karen Baker 1, Tara A. Eastwood 1, Daniel P. Mulvihill 1
1School of Biosciences, University of Kent

The present protocol describes a detailed method for the bacterial production of recombinant proteins, including typically insoluble or disulfide-bond containing proteins, packaged inside extracellular membrane-bound vesicles. This has the potential to be applied to versatile areas of scientific research, including applied biotechnology and medicine.

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Neuroscience

Neuronavigated Focalized Transcranial Direct Current Stimulation Administered During Functional Magnetic Resonance Imaging
Filip Niemann *1, Alireza Shahbabaie *1, Sven Paßmann 1, Steffen Riemann 1, Robert Malinowski 1, Harun Kocataş 1, Leonardo M. Caisachana Guevara 1,2, Mohamed Abdelmotaleb 1, Daria Antonenko 1, Felix Blankenburg 3,4, Rico Fischer 2, Gesa Hartwigsen 5,6, Shu-Chen Li 7,8, Michael A. Nitsche 9,10,11, Axel Thielscher 12,13, Dagmar Timmann 14, Anna Fromm 1, Dayana Hayek 1, Ann-Kathrin Hubert 1, Andrew K. Martin 15,16, Alexander Hunold 17, Agnes Flöel *1,18, Marcus Meinzer *1
1Department of Neurology, University Medicine Greifswald, 2Department of Psychology, University Medicine Greifswald, 3Berlin School of Mind and Brain, Humboldt Universität zu Berlin, 4Neurocomputation and Neuroimaging Unit, Freie Universität Berlin, 5Wilhelm-Wundt-Institute for Psychology, Cognitive and Biological Psychology, Leipzig University, 6Lise Meitner Research Group Cognition and Plasticity, Max Planck Institute for Human Cognitive and Brain Sciences, 7Lifespan Developmental Neuroscience, Faculty of Psychology, Technische Universität Dresden, 8Centre for Tactile Internet with Human-in-the-Loop, Technische Universität Dresden, 9Department of Psychology and Neurosciences, Leibniz Research Centre for Working Environment and Human Factors, 10German Centre for Mental Health (DZPG), 11Clinic of Psychiatry and Psychotherapy, Protestant Hospital of Bethel Foundation, University Hospital OWL, Bielefeld University, 12Danish Research Centre for Magnetic Resonance, Centre for Functional and Diagnostic Imaging and Research, Copenhagen University Hospital Amager and Hvidovre, 13Department of Health Technology, Technical University of Denmark, 14Department of Neurology and Center for Translational Neuro- and Behavioral Sciences (C-TNBS), Essen University Hospital, 15Department of Psychology, University of Kent, 16Kent Medway Medical School, 17Institute of Biomedical Engineering and Informatics, Technische Universität Ilmenau, 18German Center for Neurodegenerative Diseases (DZNE Site Greifswald)

This protocol describes the method of neuronavigated electrode placement for focal, transcranial direct current stimulation (tDCS) administered during functional magnetic resonance imaging (fMRI).

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