로그인

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.

Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the ligand-binding site. The specificity of a protein’s ligand-binding site is determined by the arrangement of its amino acid chain which gives the area its shape and chemical reactivity. Hence, a ligand-binding site provides a complementary shape to its ligand and keeps the ligand in place via chemical interactions. These chemical interactions are often noncovalent; however, since these interactions are reversible and weak, many of these interactions need to occur simultaneously to hold the protein and the ligand together.

Research that elucidates interaction mechanisms at ligand binding sites generally involves in silico modeling and in vitro approaches. In silico modeling uses computers to compare previously known protein structures and evolutionary data to make predictions to determine the optimal binding shape and energy state of the protein-ligand complex. In vitro approaches compliment in silico predictions by providing evidence for ligand binding through binding and kinetic assays in the laboratory. Ligand binding research is important for understanding the functions of proteins and how they perform specific cellular processes in both healthy, as well as in diseased conditions. For instance, certain genetic conditions and cancers can alter the sequence of a protein, ultimately affecting its ability to bind a ligand. In addition, this research also allows scientists to design drugs with specific interactions and minimal side effects by targeting the ligand-binding site of an implicated protein.

Tags

LigandBinding SitesProtein StructureMolecular InteractionsDrug DesignStructural BiologyActive SiteReceptor BindingProtein ligand InteractionsComputational Biology

장에서 6:

article

Now Playing

6.1 : Ligand Binding Sites

Protein Function

12.5K Views

article

6.2 : 단백질-단백질 인터페이스

Protein Function

3.6K Views

article

6.3 : 보존 된 바인딩 부위

Protein Function

1.6K Views

article

6.4 : 보조 인자와 코엔자임

Protein Function

10.8K Views

article

6.5 : 협력 알로스테릭 전이

Protein Function

2.3K Views

article

6.6 : 단백질 키나아제(protein kinase)와 인산분해효소(Phosphatase)

Protein Function

3.7K Views

article

6.7 : GTPase 및 해당 규정

Protein Function

2.2K Views

article

6.8 : 공유 결합 단백질 조절인자

Protein Function

1.6K Views

article

6.9 : 교체 가능한 부품을 가진 단백질 복합체

Protein Function

1.8K Views

article

6.10 : 기계적 단백질 기능

Protein Function

2.0K Views

article

6.11 : 구조적 단백질 기능

Protein Function

2.7K Views

article

6.12 : 단백질 네트워크

Protein Function

2.2K Views

JoVE Logo

개인 정보 보호

이용 약관

정책

연구

교육

JoVE 소개

Copyright © 2025 MyJoVE Corporation. 판권 소유