Immunofluorescence Microscopy of Neural Cells Differentiated from Peripheral Blood-Derived Cells

Take a multi-chamber slide with protein-coated coverslips containing fixed and permeabilized peripheral blood-derived neural cells, including neuronal progenitor cells, astrocytes, and neurons.

Add a blocking solution to block non-specific binding sites.

Incubate with fluorescent dye-conjugated antibodies that bind to specific cellular proteins, enabling the identification of neural cell types.

Wash with buffer to remove unbound antibodies.

Incubate with a fluorescent nuclear dye to stain the nuclei.

Transfer the coverslip to a slide with mounting media. Then, place the prepared slide under a fluorescence microscope equipped with appropriate filters.

Using specific wavelengths of light, excite the fluorescent dyes, causing them to emit light at longer wavelengths. 

The emitted fluorescent signals correspond to the labeled cellular proteins, distinctly highlighting different neural cell types and confirming the differentiation of reprogrammed blood cells.