This work was supported by a BU/CIMIT Applied Healthcare Engineering Predoctoral Fellowship, a National Science Foundation Broadening Participation Research Initiation Grant in Engineering (BRIGE), and the National Institutes of Health (R21EB0094930).

1. Preparation of Phase-shift Nanoemulsion (PSNE)
<ol>
 <li>Dissolve 11 mg DPPC and 1.68 mg DSPE-PEG2000 in chloroform</li>
 <li>Evaporate the organic solvent to form a dry lipid film in a glass round-bottom flask</li>
 <li>Dessicate the lipid film overnight</li>
 <li>Rehydrate the lipid film with 5.5 ml of phosphate-buffered saline (PBS)</li>
 <li>Heat solution in a 45 &deg;C water bath until lipid film dissolves, vortexing periodically</li>
 <li>Transfer lipid solution into 7 ml vial</li>
 <li>So...

<ol>
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No conflicts of interest declared.

High-intensity focused ultrasound (HIFU) is used clinically to thermally ablate tumors.1 To enhance localized heating and improve thermal ablation in tumors, lipid-coated perfluorocarbon droplets have been developed which can be vaporized by HIFU. The vasculature in many tumors is abnormally leaky due to their rapid growth.2 Thus, nanoparticles are able to penetrate the fenestrations and passively accumulate within tumors, a process known as the enhanced permeability and retention (EPR) effect...

<table border="1">
 <tbody>
 <tr>
 <td>Common Name</td>
 <td>Manufacturer</td>
 <td>Cat. Number</td>
 <td>Full Name / Description</td>
 </tr>
 <tr>
 <td>DPPC</td>
 <td>Avanti Lipids, Alabaster, AL, USA</td>
 <td>850355P</td>
 <td>1,2-dipalmitoyl-sn-glycero-3-phosphocholine</td>
 </tr>
 <tr>
 <td>DSPE-PEG2000</td>
 <td>Avanti Lipids, Alabaster, AL, USA</td>
 <td>880120P</td>
 <td>1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)</td>
 </tr>
 <tr>
 <td>DDFP</td>
 <td>Fluoromed, Round Rock, TX, USA</td>
 <td>CAS: 138495-42-8</td>
 <td>Dodecafluoropentane (C5F12)</td>
 </tr>
 <tr>
 <td>PBS</td>
 <td>Sigma-Aldrich, St. Louis, MO, USA</td>
 <td>P2194</td>
 <td>Phosphate-buffered saline</td>
 </tr>
 <tr>
 <td>Chloroform</td>
 <td>Sigma-Aldrich, St. Louis, MO, USA</td>
 <td>372978</td>
 <td>Chloroform</td>
 </tr>
 <tr>
 <td>Acrylamide</td>
 <td>Sigma-Aldrich, St. Louis, MO, USA</td>
 <td>A9926</td>
 <td>40% 19:1 acrylamide/bis-acrylamide</td>
 </tr>
 <tr>
 <td>Tris buffer</td>
 <td>Sigma-Aldrich, St. Louis, MO, USA</td>
 <td>T2694</td>
 <td>1M, pH 8, trizma hydrochloride and trizma base</td>
 </tr>
 <tr>
 <td>BSA</td>
 <td>Sigma-Aldrich, St. Louis, MO, USA</td>
 <td>A3059</td>
 <td>Bovine serum albumin</td>
 </tr>
 <tr>
 <td>APS</td>
 <td>Sigma-Aldrich, St. Louis, MO, USA</td>
 <td>A3678</td>
 <td>Ammonium persulfate solution</td>
 </tr>
 <tr>
 <td>TEMED</td>
 <td>Sigma-Aldrich, St. Louis, MO, USA</td>
 <td>87689</td>
 <td>N,N,N',N'-Tetramethylethylenediamine</td>
 </tr>
 <tr>
 <td>&nbsp;</td>
 <td>&nbsp;</td>
 <td>&nbsp;</td>
 <td>Equipment</td>
 </tr>
 <tr>
 <td>Sonicator (3 mm tip)</td>
 <td>Sonics &amp; Materials, Inc., Newtown, CT, USA</td>
 <td>Vibra-Cell</td>
 <td></td>
 </tr>
 <tr>
 <td>Water bath</td>
 <td>Thermo Fisher Scientific, Waltham, MA, USA</td>
 <td>Neslab EX-7</td>
 <td></td>
 </tr>
 <tr>
 <td>Extruder</td>
 <td>Northern Lipids, Burnaby, BC, Canada</td>
 <td>LIPEX</td>
 <td></td>
 </tr>
 <tr>
 <td>Extruder Filters</td>
 <td>Whatman, Piscataway, NJ, USA </td>
 <td>Nuclepore #110605 and #110606</td>
 <td></td>
 </tr>
 <tr>
 <td>Extruder Drain Disc</td>
 <td>Sterlitech Corporation, Kent, WA, USA</td>
 <td>#PETEDD25100</td>
 <td></td>
 </tr>
 <tr>
 <td>Plastic chamber</td>
 <td>U.S. Plastic Corporation, Lima, OH, USA</td>
 <td>#55288, 1 3/16"x1 3/16"x2 7/16"</td>
 <td></td>
 </tr>
 </tbody>
</table>

synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation

High-intensity focused ultrasound (HIFU) is used clinically to thermally ablate tumors. To enhance localized heating and improve thermal ablation in tumors, lipid-coated perfluorocarbon droplets have been developed which can be vaporized by HIFU. The vasculature in many tumors is abnormally leaky due to their rapid growth, and nanoparticles are able to penetrate the fenestrations and passively accumulate within tumors. Thus, controlling the size of the droplets can result in better accumulation within tumors. In this report, the preparation of stable droplets in a phase-shift nanoemulsion (PSNE) with a narrow size distribution is described. PSNE were synthesized by sonicating a lipid solution in the presence of liquid perfluorocarbon. A narrow size distribution was obtained by extruding the PSNE multiple times using filters with pore sizes of 100 or 200 nm. The size distribution was measured over a 7-day period using dynamic light scattering. Polyacrylamide hydrogels containing PSNE were prepared for in vitro experiments. PSNE droplets in the hydrogels were vaporized with ultrasound and the resulting bubbles enhanced localized heating. Vaporized PSNE enables more rapid heating and also reduces the ultrasound intensity needed for thermal ablation. Thus, PSNE is expected to enhance thermal ablation in tumors, potentially improving therapeutic outcomes of HIFU-mediated thermal ablation treatments.

Watch this Scientific Journal Video about Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation at Jo

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation

Phase-shift nanoemulsions (PSNE) can be vaporized using high intensity focused ultrasound to enhance localized heating and improve thermal ablation in tumors. In this report, the preparation of stable PSNE with a narrow size distribution is described. Furthermore, the impact of vaporized PSNE on ultrasound-mediated ablation is demonstrated in tissue-mimicking phantoms.

High-intensity  focused ultrasound (HIFU) is used clinically to thermally ablate tumors. To  enhance localized heating and improve thermal ablation in ...

synthesis-phase-shift-nanoemulsions-with-narrow-size-distributions

Research

JoVE Journal

Engineering

10.7K 조회수.  Boston University. 위상 시프트 nanoemulsions (PSNE)는 지역화 된 난방을 개선하고 종양의 열 절제를 개선하기 위해 높은 강도 집중 초음파를 사용하여 기화 할 수 있습니다. 이 보고서에서는 좁은 크기 분포와 안정 PSNE의 준비가 설명되어 있습니다. 또한, 초음파로 인한 박리에 기화의 PSNE의 영향은 조직 - 모방의 유령에서 증명된다.

비디오: Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation