이 문서에 포함 된 작품은 미국의 건강 보조 재단 (XL), 실명을 방지하기 위해 연구에서 윌리엄 &amp; 메리 그레베 특별 장학생 수상 (XL)에서 녹내장 연구 박사 더글러스 H. 존슨 수상에 의해 지원되었다 실명 예방 일리노이 학회 (HC)와 NIH 부여 R01EY019034 (XL).

절차 C57BL/6J 마우스 (잭슨 연구소, 바 하버, 메인)은 노스 웨스턴 대학교의 동물 관리 시설에서 발생합니다. 모든 동물은 노스 웨스턴 대학 기관 애니멀 케어 및 사용위원회와 NIH에서 신경 과학 연구에서 동물의 사용에 대한 지침을 따른다 승인 프로토콜에 따라 사용됩니다. 1. 레이저 광응고술 레이저 광응고술의 절차는 5-7 이전에 발행 된 프로토콜에서 수정됩니다. <ol><li> 케타민 (100 ㎎ / ㎏, 버틀러 Schein의 동물 건강, OH)과 xylazine (아이오와, 셰넌 도어의 10 ㎎ / ㎏, 로이드 주식, IA)의 복강 주사로 40 ~ 60 일 이전 마우스를 마취. </li><li> 1 % 아트로핀 황산염 솔루션의 하나 또는 두 방울 (알콘 연구소, 주식 회사, 포트 워스, 텍사스)와 국소 치료로 실험 동물의 오른쪽 눈의 동공을 팽창. </li><li> 산동 후, 평평하게nterior 챔버 레이저 유도는 6 강화한다. 전방 액을 배출 슬릿 램프 아래 전방 공간 (SL-3E, 탑콘, 오클랜드, NJ)에 예리한 끝 (세계 정밀 계측기 Inc의 새러 소타, 플로리다)와 유리 마이크로 피펫을 삽입합니다. </li><li> 플라스틱 콘 홀더 (브레인 과학 주식 회사, MA)에 마우스를 억제하고 (그림 1A 참조) 만든 플랫폼에 묶여. 고정 장치와 마우스를 잡고 슬릿 램프 뒤에 광원 마우스의 오른쪽 눈을 노출합니다. 슬릿 램프에서 마취 마우스의 오른쪽 눈을 맞 춥니 다. </li><li> 두 손으로 마우스 고정 장치를 잡고있는 동안, 아르곤 레이저 (울티마 2000SE, 일관된, 산타 클라라, CA)를 사용하여 각막 윤부에 레이저 조명을 적용합니다. 수직 섬유주의 둘레 80-100 레이저 반점 (514 nm의, 100 mW의 50 밀리 초 펄스, 200 μm의 지점)에 대해 제공합니다. C57BL / 6 마​​우스는 P에 대한 장벽 역할을 색칠 조리개를 가지고otential 길잃은 에너지 7. </li><li> 주입 국소 0.5 % 목시 플록 사신 (알콘 연구소, 주식 회사, 포트 워스, 텍사스) 레이저 치료 부위와 0.5 %가 Proparacaine이 (바슈롬, 로체스터, NY) 통증을 완화하는 소독하는 안구 표면에.에게 </li><li> 완전히 깨어 때까지 약 1 시간 동안 복구를 위해 가열 패드 (태양열 제품 Inc, 보카 레이 톤, 플로리다)에 동물을 유지합니다. </li><li> 왼쪽 눈은 대조군으로 봉사하는 치료입니다. </li></ol> 2. 안압 측정 <ol><li> 플라스틱 콘 홀더에로드 한 다음 (그림 2A 참조) 플랫폼을 억제하는 튜브에 깨어 마우스를 놓습니다. </li><li> 시간을 5 분 내지는 10 분 마우스 홀더의 위치에 적응하자 할 수 있습니다. 프로브 팁이 떨어져 각막 (14)의 표면 2~3밀리미터 때까지 마우스 눈 리바운드 안압계 (TonoLab, 콜로니얼 의료 공급, 프란 코니아, 뉴햄프셔) 접근. </li><li> 프로브 팁의 중심 표면을 칠 수 있도록 측정 버튼을 눌러부드럽게의 각막. 같은 눈의 안압 여섯 측정 연속 3 세트 취득하고 눈의 안압으로 평균입니다. 치료 컨트롤의 눈은 항상 다음 측정 레이저 치료 눈을위한 기준 수치를 얻기 위해 먼저 측정됩니다. </li></ol> 3. Optomotor 테스트 시력 및 대비 감도는 14,15을 테스트합니다. 개인 마우스의 두 눈 표류 격자의 방향을 반대로하여 개별적으로 검토되며, 시계 방향으로 표류 격자가 왼쪽 눈과 오른쪽 눈 16 반 시계 방향으로 표류 격자의 시각적 기능을 식별하는 데 사용됩니다 즉. 각 테스트는 약 15 분 정도 소요 독립적으로 두 관찰자에 의해 반복됩니다. <ol><li> 마우스를 놓고 마우스가 네 개의 컴퓨터 모니터 (그림 3A-B)으로 둘러싸인 높은 플랫폼에서 자유롭게 이동할 수 있습니다. </li><li> 그들은 수평 사인 표류 표시되는 있도록 모니터를 설정39 CD / 평방 미터의 평균 휘도 시각적 자극으로 격자. 격자의 이동 방향은 교대로한다 연속적 사이에 시계 방향 및 반 시계 방향으로. </li><li> 동물의 움직임을 분석합니다. 시각적 인 자극에 다음 점차적으로 증가 후 표류 격자와 함께 콘서트에서 동물의 움직임을 15 초 내에 &quot;긍정적&quot;으로 간주됩니다. 가장 높은 응답-이끌어내는 시각적 자극은 동물의 시력이 17로 정의됩니다. </li><li> 0.075, 0.16, 및 학위 당 0.3 회 (CPD) : 세 개의 미리 설정된 공간 주파수에서 대비 감도를 검사합니다. 각각의 눈에 대한 대비 임계 값은 미리 고정 주파수에서 시각적 인 반응을 이끌어 낮은 대비로 정의됩니다. 대비 감도는 임계 값 17의 역수이다. </li></ol>

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저자는 그들이 더 경쟁 재정적 이익이 없다는 것을 선언합니다. 저자는 노스 웨스턴 대학의 풀 타임 직원입니다. 저자는이 문서에 사용 된 시약 및 장비를 생산하는 업체가 제공 한 어떠한 자금을받지 않았다.

우리가 지속적인 안압 위의 보고서는 마우스 눈에서 레이저 조명에 의해 유도 될 수있다. 식염수 주입 모델 18 정맥 소작 모델 광범위한 미세 기술을 필요로 둘 다 11에 비해, 레이저 조명 수행 할 비교적 간단하고 쉽습니다. 보통 우리는 2-3 시간에서 4-6 생쥐 레이저 조명을 수행 할 수 있습니다. 지속적인 안압 상승을 달성하기 위해 중요한 단계 레이저 조명 레이저 매개 변수 앞에 ...

<table><tbody><tr><td colspan="4">&lt;strong&gt;Reagent&lt;/strong&gt;</td></tr><tr><td>moxifloxacin</td><td>Alcon Labs, Inc.</td><td>NDC 0065-4013-03</td><td>0.5 %, Rx only</td></tr><tr><td>Proparacaine Hydrochloride</td><td>Bausch Lomb</td><td>NDC 24208-730-06</td><td>0.5 %, Rx only</td></tr><tr><td>Ophthalmic Solution USP</td><td>Bausch Lomb</td><td>NDC 24208-730-06</td><td>.5 %, Rx only</td></tr><tr><td>ketamine</td><td>Butler Schein Animal Health</td><td>NDC 11695-0550-1</td><td>100 mg / kg </td></tr><tr><td>xylazine</td><td>LLOYD Inc. of Iowa</td><td>NADA 139-236</td><td>10 mg / kg </td></tr><tr><td>atropine sulfate solution</td><td>Alcon Labs, Inc.</td><td>NDC 61314-303-02</td><td>1 %, Rx only</td></tr><tr><td colspan="4">&lt;strong&gt;Equipment &lt;/strong&gt;</td></tr><tr><td>Slit Lamp, TOPCON </td><td>Visual Systems Inc</td><td>SL-3E</td><td>powered by PS-30A</td></tr><tr><td>OptoMotry 1.8.0 virtual</td><td>CerebralMechanics Inc.</td><td></td><td></td></tr><tr><td>opto-kinetic testing system</td><td>CerebralMechanics Inc.</td><td></td><td></td></tr><tr><td>Tonometer, TonoLab, for mice</td><td>Colonial Medical Supply</td><td></td><td></td></tr><tr><td>Heating pad</td><td>Sunbeam Products Inc</td><td>722-810</td><td></td></tr><tr><td>Argon laser </td><td>Coherent Inc</td><td>Ultima 2000SE</td><td></td></tr><tr><td>DECAPICONE Plastic cone holder </td><td>Braintree Sci Inc.</td><td>MDC-200</td><td>for mouse</td></tr></tbody></table>

a laser-induced mouse model of chronic ocular hypertension to characterize visual defects

자주 높은 안압 (IOP)와 관련된 녹내장은 실명의 주요 원인 중 하나입니다. 우리는 인간의 높은 안압 녹내장을 모방하는 안구 고혈압의 마우스 모델을 구축하기 위해 노력했다. 여기에 레이저 조명은 각 폐쇄를 유도 수성 유출 photocoagulate하기 위해 각막 윤부에 적용됩니다. IOP의 변화는 레이저 치료 전후 리바운드 안압계를 사용하여 모니터링 할 수 있습니다. optomotor 행동 테스트는 Visual 용량의 해당 변화를 측정하는 데 사용됩니다. 레이저 조명 후 지속적인 안압 상승을 개발 한 마우스에서 대표적인 결과가 표시됩니다. 시력 저하 및 대비 감도는이 안구 고혈압 쥐에서 관찰된다. 함께, 우리의 연구는 녹내장 쥐에서 신경 세포의 변성 및 기본 분자 메커니즘을 조사하기 위해 중요한 모델 시스템을 소개합니다.

절차에서 설명한대로, 레이저 조명은 폐쇄 각 (그림 1)을 유도, 수성 유출 photocoagulate하는 각막 윤부 지역에서 섬유주위한 것입니다. 대부분의 레이저로 눈 앞의 연구 결과 6 일치 유의 물리적 손상, 색소 분리 또는 감염을 전시하지 않습니다. 쥐의 작은 그룹 (모든 레이저로 동물의 5 % 미만)은 수축 눈 공, 심한 백내장, 중요한 색소 분리, 또는 출혈 등 심각한 손상의 물리적 흔?...

Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects

만성 안구 고혈압 쥐의 눈 섬유주 레이저 광응고술을 사용하여 유도된다. 안압 (IOP)는 레이저 치료 후 몇 개월 동안 상승합니다. 시력 실험 동물의 대비 감도의 감소는 optomotor 테스트를 사용하여 모니터링 할 수 있습니다.

비주얼 결함의 특성을 만성 안구 고혈압의 레이저 유도 마우스 모델

Glaucoma, frequently associated with elevated intraocular pressure (IOP), is one of the leading causes of blindness. We sought to establish a mouse model ...

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Research

JoVE Journal

Medicine

12.9K 조회수.  Northwestern University. 만성 안구 고혈압 쥐의 눈 섬유주 레이저 광응고술을 사용하여 유도된다. 안압 (IOP)는 레이저 치료 후 몇 개월 동안 상승합니다. 시력 실험 동물의 대비 감도의 감소는 optomotor 테스트를 사용하여 모니터링 할 수 있습니다.

비디오: A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects

Laser-Induced Chronic Ocular Hypertension Model on SD Rats

Glaucoma is one of the major causes of blindness in the world. Elevated intraocular pressure is a major risk factor. Laser photocoagulation induced ocular hypertension is one of the well established animal models. This video demonstrates how to induce ocular hypertension by Argon laser photocoagulation in rat.

Glaucoma is one of the major causes of blindness in the world. Elevated intraocular pressure is a major risk factor. Laser photocoagulation induced ocular hypertension is one of the well established animal models. This video demonstrates how to induce ocular hypertension by Argon laser photocoagulation in rat.

SD 쥐에서 레이저 유도 만성 안구 고혈압 모델

Glaucoma is one of the major causes of blindness in the world. Elevated intraocular pressure is a major risk factor. Laser photocoagulation induced ocular ...

연구

생물학

A Mouse Model for Laser-induced Choroidal Neovascularization

The mouse laser-induced choroidal neovascularization (CNV) model has been a crucial mainstay model for neovascular age-related macular degeneration (AMD) research. By administering targeted laser injury to the RPE and Bruch&#8217;s membrane, the procedure induces angiogenesis, modeling the hallmark pathology observed in neovascular AMD. 
First developed in non-human primates, the laser-induced CNV model has come to be implemented into many other species, the most recent of which being the mouse. Mouse experiments are advantageously more cost-effective, experiments can be executed on a much faster timeline, and they allow the use of various transgenic models. The miniature size of the mouse eye, however, poses a particular challenge when performing the procedure. Manipulation of the eye to visualize the retina requires practice of fine dexterity skills as well as simultaneous hand-eye-foot coordination to operate the laser. However, once mastered, the model can be applied to study many aspects of neovascular AMD such as molecular mechanisms, the effect of genetic manipulations, and drug treatment effects. 
The laser-induced CNV model, though useful, is not a perfect model of the disease. The wild-type mouse eye is otherwise healthy, and the chorio-retinal environment does not mimic the pathologic changes in human AMD. Furthermore, injury-induced angiogenesis does not reflect the same pathways as angiogenesis occurring in an age-related and chronic disease state as in AMD.
Despite its shortcomings, the laser-induced CNV model is one of the best methods currently available to study the debilitating pathology of neovascular AMD. Its implementation has led to a deeper understanding of the pathogenesis of AMD, as well as contributing to the development of many of the AMD therapies currently available.

Here, we present the mouse laser-induced choroidal neovascularization (CNV) protocol, an experimental model that re-creates the vascular hallmarks of neovascular age-related macular degeneration (AMD). Once mastered, it can reliably and effectively induce CNV as a model system to test various experimental measures.

레이저에 의한 맥락막 신생 혈관에 대한 마우스 모델

The mouse laser-induced choroidal neovascularization (CNV) model has been a crucial mainstay model for neovascular age-related macular degeneration (AMD) ...

의학

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

The use of rodent models of glaucoma has been essential to understand the molecular mechanisms that underlie the pathophysiology of this multifactorial neurodegenerative disease. With the advent of numerous transgenic mouse lines, there is increasing interest in inducible murine models of ocular hypertension. Here, we present an occlusion model of glaucoma based on the injection of magnetic microbeads into the anterior chamber of the eye using a modified microneedle with a facetted bevel. The magnetic microbeads are attracted to the iridocorneal angle using a handheld magnet to block the drainage of aqueous humour from the anterior chamber. This disruption in aqueous dynamics results in a steady elevation of intraocular pressure, which subsequently leads to the loss of retinal ganglion cells, as observed in human glaucoma patients. The microbead occlusion model presented in this manuscript is simple compared to other inducible models of glaucoma and also highly effective and reproducible. Importantly, the modifications presented here minimize common issues that often arise in occlusion models. First, the use of a bevelled glass microneedle prevents backflow of microbeads and ensures that minimal damage occurs to the cornea during the injection, thus reducing injury-related effects. Second, the use of magnetic microbeads ensures the ability to attract most beads to the iridocorneal angle, effectively reducing the number of beads floating in the anterior chamber avoiding contact with other structures (e.g., iris, lens). Lastly, the use of a handheld magnet allows flexibility when handling the small mouse eye to efficiently direct the magnetic microbeads and ensure that there is little reflux of the microbeads from the eye when the microneedle is withdrawn. In summary, the microbead occlusion mouse model presented here is a powerful investigative tool to study neurodegenerative changes that occur during the onset and progression of glaucoma.

Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous humour.

자기 마이크로 비드 폐색 모델 마우스의 녹내장 안구 고혈압 종속 유도합니다

The use of rodent models of glaucoma has been essential to understand the molecular mechanisms that underlie the pathophysiology of this multifactorial ...

In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model

Laser-induced choroidal neovascularization (CNV) is a well-established model to mimic the wet form of age-related macular degeneration (AMD). In this protocol, we aim to guide the reader not simply through the technical considerations of generating laser-induced lesions to trigger neovascular processes, but rather focus on the powerful information that can be obtained from multimodal longitudinal in vivo imaging throughout the follow-up period.
The laser-induced mouse CNV model was generated by a diode laser administration. Multimodal in vivo imaging techniques were used to monitor CNV induction, progression and regression. First, spectral domain optical coherence tomography (SD-OCT) was performed immediately after the lasering to verify a break of Bruch&#39;s membrane. Subsequent in vivo imaging using fluorescein angiography (FA) confirmed successful damage of Bruch&#39;s membrane from serial images acquired at the choroidal level. Longitudinal follow-up of CNV proliferation and regression on days 5, 10, and 14 after the lasering was performed using both SD-OCT and FA. Simple and reliable grading of leaky CNV leasions from FA images is&#160;presented. Automated segmentation for measurement of total retinal thickness, combined with manual caliber application for measurement of retinal thickness at CNV sites, allow unbiased evaluation of the presence of edema. Finally, histological verification of CNV is performed using isolectin GS-IB4 staining on choroidal flatmounts. The staining is thresholded, and the isolectin-positive area is calculated with ImageJ.
This protocol is especially useful in therapeutics studies requiring high-throughput-like screening of CNV pathology as it allows fast, multimodal, and reliable classification of CNV pathology and retinal edema. In addition, high resolution SD-OCT enables the recording of other pathological hallmarks, such as the accumulation of subretinal or intraretinal fluid. However, this method does not provide a possibility to automate CNV volume analysis from SD-OCT images, which has to be performed manually.

In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model

Here, we present the usefulness of longitudinal in vivo imaging in the follow-up of morphological changes of laser-induced choroidal neovascularization in mice.

Vivo에서 복합 이미징 및 마우스 레이저 유도 안 Neovascularization 모델의 분석

Laser-induced choroidal neovascularization (CNV) is a well-established model to mimic the wet form of age-related macular degeneration (AMD). In this ...

신경과학

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Spectral-domain optical coherence tomography (SD-OCT) is useful for visualizing retinal and ocular structures in vivo. In research, SD-OCT is a valuable tool to evaluate and characterize changes in a variety of retinal and ocular disease and injury models. In light induced retinal degeneration models, SD-OCT can be used to track thinning of the photoreceptor layer over time. In glaucoma models, SD-OCT can be used to monitor decreased retinal nerve fiber layer and total retinal thickness and to observe optic nerve cupping after inducing ocular hypertension. In diabetic rodents, SD-OCT has helped researchers observe decreased total retinal thickness as well as decreased thickness of specific retinal layers, particularly the retinal nerve fiber layer with disease progression. In mouse models of myopia, SD-OCT can be used to evaluate axial parameters, such as axial length changes. Advantages of SD-OCT include in vivo imaging of ocular structures, the ability to quantitatively track changes in ocular dimensions over time, and its rapid scanning speed and high resolution. Here, we detail the methods of SD-OCT and show examples of its use in our laboratory in models of retinal degeneration, glaucoma, diabetic retinopathy, and myopia. Methods include anesthesia, SD-OCT imaging, and processing of the images for thickness measurements.

Here, we describe the use of spectral-domain optical coherence tomography (SD-OCT) to visualize retinal and ocular structures in vivo in models of retinal degeneration, glaucoma, diabetic retinopathy, and myopia.

광학 간섭 단층 촬영을 사용한 설치류 모델에서 안구 질환의 생체 내 구조 평가

Spectral-domain optical coherence tomography (SD-OCT) is useful for visualizing retinal and ocular structures in vivo. In research, SD-OCT is a valuable ...

생체공학

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

The circumlimbal suture is a technique for inducing experimental glaucoma in rodents by chronically elevating intraocular pressure (IOP), a well-known risk factor for glaucoma. This protocol demonstrates a step-by-step guide on this technique in Long Evans rats and C57BL/6 mice. Under general anesthesia, a "purse-string" suture is applied on the conjunctiva, around the equator and behind the limbus of the eye. The fellow eye serves as an untreated control. Over the duration of our study, which was a period of 8 weeks for rats and 12 weeks for mice, IOP remained elevated, as measured regularly by rebound tonometry in conscious animals without topical anesthesia. In both species, the sutured eyes showed electroretinogram features consistent with preferential inner retinal dysfunction. Optical coherence tomography showed selective thinning of the retinal nerve fiber layer. Histology of the rat retina in cross-section found reduced cell density in the ganglion cell layer, but no change in other cellular layers. Staining of flat-mounted mouse retinae with a ganglion cell specific marker (RBPMS) confirmed ganglion cell loss. The circumlimbal suture is a simple, minimally invasive and cost-effective way to induce ocular hypertension that leads to ganglion cell injury in both rats and mice.

Chronic ocular hypertension is induced by applying a circumlimbal suture in rats and mice, leading to functional and structural deterioration of the retinal ganglion cells consistent with glaucoma.

쥐와 쥐에 Circumlimbal 봉합에 의해 유도 된 녹 내장의 모델

The circumlimbal suture is a technique for inducing experimental glaucoma in rodents by chronically elevating intraocular pressure (IOP), a well-known ...

Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability

Mouse models of retinal vein occlusion (RVO) are often used in ophthalmology to study hypoxic-ischemic injury in the neural retina. In this report, a detailed method pointing out critical steps is provided with recommendations for optimization to achieve consistently successful occlusion rates across different genetically modified mouse strains. The RVO mouse model consists primarily of the intravenous administration of a photosensitizer dye followed by laser photocoagulation using a retinal imaging microscope attached to an ophthalmic guided laser. Three variables were identified as determinants of occlusion consistency. By adjusting the wait time after rose bengal administration and balancing the baseline and experimental laser output, the variability across experiments can be limited and a higher success rate of occlusions achieved. This method can be used to study retinal diseases that are characterized by retinal edema and hypoxic-ischemic injury. Additionally, as this model induces vascular injury, it can also be applied to study the neurovasculature, neuronal death, and inflammation.

Here, we describe an optimized protocol for retinal vein occlusion using rose bengal and a laser-guided retinal imaging microscope system with recommendations to maximize its reproducibility in genetically modified strains.

가변성을 제한하기 위한 망막 정맥 폐색 마우스 모델의 최적화

Mouse models of retinal vein occlusion (RVO) are often used in ophthalmology to study hypoxic-ischemic injury in the neural retina. In this report, a ...

Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents

Glaucoma, the second leading cause of blindness worldwide, is a heterogeneous group of ocular disorders characterized by structural damage to the optic nerve and retinal ganglion cell (RGC) degeneration, resulting in visual dysfunction by interrupting the transmission of visual information from the eye to the brain. Elevated intraocular pressure is the most important risk factor; thus, several models of ocular hypertension have been developed in rodents by either genetic or experimental approaches to investigate the causes and effects of the disease. Among those, some limitations have been reported such as surgical invasiveness, inadequate functional assessment, requirement of extensive training, and highly variable extension of retinal damage. The present work characterizes a simple, low-cost, and efficient method to induce ocular hypertension in rodents, based on low-temperature, full-circle cauterization of the limbal vascular plexus, a major component of aqueous humor drainage. The new model provides a technically easy, noninvasive, and reproducible subacute ocular hypertension, associated with progressive RGC and optic nerve degeneration, and a unique post-operative clinical recovery rate that allows in vivo functional studies by both electrophysiological and behavioral methods.

The goal of this protocol is to characterize a novel model of glaucomatous neurodegeneration based on 360&#176; thermic cauterization of limbal vascular plexus, inducing subacute ocular hypertension.

설치류의 외과적으로 유도된 녹내장에 대한 Limbal Vascular Plexus의 전체 원형 소작

Glaucoma, the second leading cause of blindness worldwide, is a heterogeneous group of ocular disorders characterized by structural damage to the optic ...

마우스 Retina OCT Fibergram Alignment with Confocal Images

Alignment of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina

In recent years, in vivo retinal imaging, which provides non-invasive, real-time, and longitudinal information about biological systems and processes, has been increasingly applied to obtain an objective assessment of neural damage in eye diseases. Ex vivo confocal imaging of the same retina is often necessary to validate the in vivo findings especially in animal research. In this study, we demonstrated a method for aligning an ex vivo confocal image of the mouse retina with its in vivo images. A new clinical-ready imaging technology called visible light optical coherence tomography fibergraphy (vis-OCTF) was applied to acquire in vivo images of the mouse retina. We then performed the confocal imaging of the same retina as the &#34;gold standard&#34; to validate the in vivo vis-OCTF images. This study not only enables further investigation of the molecular and cellular mechanisms but also establishes a foundation for a sensitive and objective evaluation of neural damage in vivo.

저자 스포트라이트: 녹내장 진단 및 치료 개선을 위한 In Vivo 및 Ex Vivo 망막 영상의 발전 

The present protocol outlines the steps for aligning in vivo visible-light optical coherence tomography fibergraphy (vis-OCTF) images with ex vivo confocal images of the same mouse retina for the purpose of verifying the observed retinal ganglion cell axon bundle morphology in the in vivo images.

Align of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina(가시광선 광간섭 단층 촬영 파이버그램과 동일한 마우스 망막의 컨포칼 이미지 정렬)

In recent years, in vivo retinal imaging, which provides non-invasive, real-time, and longitudinal information about biological systems and processes, has ...

비주얼 결함의 특성을 만성 안구 고혈압의 레이저 유도 마우스 모델

요약

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SD 쥐에서 레이저 유도 만성 안구 고혈압 모델

레이저에 의한 맥락막 신생 혈관에 대한 마우스 모델

자기 마이크로 비드 폐색 모델 마우스의 녹내장 안구 고혈압 종속 유도합니다

Vivo에서 복합 이미징 및 마우스 레이저 유도 안 Neovascularization 모델의 분석

광학 간섭 단층 촬영을 사용한 설치류 모델에서 안구 질환의 생체 내 구조 평가

쥐와 쥐에 Circumlimbal 봉합에 의해 유도 된 녹 내장의 모델

가변성을 제한하기 위한 망막 정맥 폐색 마우스 모델의 최적화

설치류의 외과적으로 유도된 녹내장에 대한 Limbal Vascular Plexus의 전체 원형 소작

Align of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina(가시광선 광간섭 단층 촬영 파이버그램과 동일한 마우스 망막의 컨포칼 이미지 정렬)

Align of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina(가시광선 광간섭 단층 촬영 파이버그램과 동일한 마우스 망막의 컨포칼 이미지 정렬)