Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
May 25th, 2015
•Luminescent identification of functional elements in 3’ untranslated regions (3’UTRs) (3’LIFE) is a technique to identify functional regulation in 3’UTRs by miRNAs or other regulatory factors. This protocol utilizes high-throughput methodology such as 96-well transfection and luciferase assays to screen hundreds of putative interactions for functional repression.
Related Videos
High-throughput Saccharification Assay for Lignocellulosic Materials
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
RNA Secondary Structure Prediction Using High-throughput SHAPE
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
Identification of Kinase-substrate Pairs Using High Throughput Screening
High Throughput Danio Rerio Energy Expenditure Assay
Copyright © 2024 MyJoVE Corporation. 판권 소유