Murine Femur and Tibia Single Bone Marrow Suspesion Preparation
5:55
Bone Marrow Cell (BMC) Culture
8:39
Results: Representative Bone Marrow Dendritic Cell Culture Analyses
10:13
Conclusion
필기록
The overall goal is this procedure is to generate and distinguish alveolar-like macrophages from dendritic cells in in vitro murine bone marrow cell cultures supplemented with GM-CSF. This in vitro method can help answer key questions regarding th
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Bone marrow cells cultured with granulocyte macrophage colony stimulating factor (GM-CSF) generate a heterogeneous culture containing macrophages and dendritic cells (DCs). This method highlights using MHCII and hyaluronan (HA) binding to differentiate macrophages from the DCs in the GM-CSF culture. Macrophages in this culture have many similarities to alveolar macrophages.