Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

DOI :

10.3791/54294-v

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September 25th, 2016

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9,560 Views

Dong-Eog Kim¹, Jeong-Yeon Kim¹, Su-Kyoung Lee¹, Ju Hee Ryu², Ick Chan kwon², Cheol-Hee Ahn³, Kwangmeyung Kim², Dawid Schellingerhout⁴

¹Molecular Imaging and Neurovascular Research Laboratory, Dongguk University College of Medicine, ²Biomedical Research Center, Korea Institute of Science and Technology, ³Research Institute of Advanced Materials, Department of Materials Science and Engineering, Seoul National University, ⁴Departments of Radiology and Cancer Systems Imaging, University of Texas M.D. Anderson Cancer Center

Chapters

This protocol describes the application of combined near-infrared fluorescent (NIRF) imaging and micro-computed tomography (microCT) for visualizing cerebral thromboemboli. This technique allows the quantification of thrombus burden and evolution. The NIRF imaging technique visualizes fluorescently labeled thrombus in excised brain, while the microCT technique visualizes thrombus inside living animals using gold-nanoparticles.

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