Growth of Microengineered Intestinal Villi in the Gut-on-a-Chip Device
3:45
Formation of Microengineered Caco-2 Villi in the Gut-on-a-Chip
5:55
Application of Mechanical Deformations Mimicking Peristalsis-like Motions
6:37
Host-gut Microbiome Co-culture in a Gut-on-a-chip Microdevice
9:07
Results: Emulating the Human Intestinal Host-microbiome Ecosystem
9:44
Conclusion
필기록
This overall goal of this protocol is to demonstrate the villus growth of human intestinal cells under peristalsis-like motions and flow in a gut-on-a-chip microfluidic device, as well as to show how to co-culture these cells with living gut micro
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We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.