November 10th, 2016
•Targeted DNA damage can be achieved by tethering a DNA damaging agent to a triplex-forming oligonucleotide (TFO). Using modified TFOs, DNA damage-specific protein association, and DNA topology modification can be studied in human cells by the utilization of modified chromatin immunoprecipitation assays and DNA supercoiling assays described herein.
Related Videos
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
Methodology for Accurate Detection of Mitochondrial DNA Methylation
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
Copyright © 2024 MyJoVE Corporation. 판권 소유