March 5th, 2018
•The goal of this protocol is to quantify the repair of defined DNA-protein crosslinks on plasmid DNA. Lesioned plasmids are transfected into recipient mammalian cell lines and low-molecular weight harvested at multiple time points post-transfection. DNA repair kinetics are quantified using strand-specific primer extension followed by qPCR.
Related Videos
Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Copyright © 2024 MyJoVE Corporation. 판권 소유