The overall goal of this experiment is to isolate quiescent neutrophils and measure their DNA release in response to agonists. This method can help answer key questions in immunology and veterinary medicine such as, what is the neutrophil response
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.