Generating cDNA from Isolated RNA by Reverse Transcription and Analysis of Cells by Quantitative PCR
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Results: Imflammatory Response in Mouse Primary Hepatocyte Cultures
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Conclusion
필기록
The overall goal of this procedure is to successfully isolate functional murine hepatocytes for studying the expression and secretion of inflammatory cytokines such as CRP and IL6 in response to mediators of the hepatic acute phase response. This
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Here, we show an enzymatic approach to isolate primary hepatocytes from adult mice, and we describe the quantification of an inflammatory response using ELISA and real-time PCR.