Sample Preparation and Amplification of CRISPER/Cas9 Regions with Fluorescent PCR
4:32
Capillary Gel Electrophoresis and Electropherogram Analysis
6:25
Results: Genotyping HEPG2 Targeted with CRISPER/Cas9 Construct Against NAP1L1
7:27
Conclusion
필기록
The overall goal of this technique is to genotype CRISPR/Cas9 induced insertion deletion mutant clones in a high-throughput manner. This method can help answer key questions in the genetics field, such as the phenotypic effect of knocking out a pa
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The genotyping technique described here, which couples fluorescent polymerase chain reaction (PCR) to capillary gel electrophoresis, allows for high-throughput genotyping of nuclease-mediated knockout clones. It circumvents limitations faced by other genotyping techniques and is more cost effective than sequencing methods.