Assessment of Binding Between Notch Receptor and Trans-ligands
1:47
Aggregation Between Signal-sending and Signal-receiving Cells
3:11
Evaluation of Inhibition of Binding Between Notch and Trans-ligands by Cis-ligands
4:15
Results: Faithful Analysis of Notch Signaling
5:30
Conclusion
필기록
The overall goal of this aggregation assay is to assess the binding of Notch receptor with trans-ligands and its inhibition by cis-ligands. This method can help answer a key question, How will genetic or pharmacological factor affect the binding o
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Complexity of in vivo systems makes it difficult to distinguish between the activation and inhibition of Notch receptor by trans- and cis-ligands, respectively. Here, we present a protocol based on in vitro cell-aggregation assays for qualitative and semi-quantitative evaluation of the binding of Drosophila Notch to trans-ligands vs cis-ligands.