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In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

24.9K Views

10:07 min

November 13th, 2014

DOI :

10.3791/51943-v

November 13th, 2014


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Keywords MRNA Synthesis

Hoofdstukken in deze video

0:05

Title

1:35

Amplification of Plasmid Inserts and Addition of Poly T-tail by PCR

2:48

In Vitro Transcription

3:59

Treatment of Purified mRNA with Antartic Phosphatase

5:17

mRNA Transfection of Cells

7:01

Flow Cytometric Analyses of eGFP Expression in Cells

8:14

Results: Modified mRNA is Expressed in Human Cells

9:34

Conclusion

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