Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

Podsumowanie

A protocol for the protein quantification in complex biological fluids using automated immuno-MALDI (iMALDI) technology is presented.

Streszczenie

Mass spectrometry (MS) is one of the most commonly used technologies for quantifying proteins in complex samples, with excellent assay specificity as a result of the direct detection of the mass-to-charge ratio of each target molecule. However, MS-based proteomics, like most other analytical techniques, has a bias towards measuring high-abundance analytes, so it is challenging to achieve detection limits of low ng/mL or pg/mL in complex samples, and this is the concentration range for many disease-relevant proteins in biofluids such as human plasma. To assist in the detection of low-abundance analytes, immuno-enrichment has been integrated into the assay to concentrate and purify the analyte before MS measurement, significantly improving assay sensitivity. In this work, the immuno- Matrix-Assisted Laser Desorption/Ionization (iMALDI) technology is presented for the quantification of proteins and peptides in biofluids, based on immuno-enrichment on beads, followed by MALDI-MS measurement without prior elution. The anti-peptide antibodies are functionalized on magnetic beads, and incubated with samples. After washing, the beads are directly transferred onto a MALDI target plate, and the signals are measured by a MALDI-Time of Flight (MALDI-TOF) instrument after the matrix solution has been applied to the beads. The sample preparation procedure is simplified compared to other immuno-MS assays, and the MALDI measurement is fast. The whole sample preparation is automated with a liquid handling system, with improved assay reproducibility and higher throughput. In this article, the iMALDI assay is used for determining the peptide angiotensin I (Ang I) concentration in plasma, which is used clinically as readout of plasma renin activity for the screening of primary aldosteronism (PA).

Wprowadzenie

Mass spectrometry has become an indispensable tool in quantitative proteomics. Mass spectrometry can determine the masses of target proteins or peptides, therefore the obtained analyte signals can be highly specific compared to immunoassays. Two ionization methods, electrospray and MALDI, are most commonly used for detecting proteins and peptides^1,2,3,4. A major challenge in MS-based protein quantification lies in the detection of low-abundance proteins in complex samples at ng/mL or pg/mL concentrations in the presence of high-abundance proteins, and many candidate protein biomarkers found in human plasma are within this range⁵. This problem is largely caused by the inherently wide dynamic range and complexity of the human proteome⁶.

To overcome these detection challenges, immuno-MS methods have been developed to enrich the target proteins or peptides from the sample solutions onto a solid surface, followed by elution of the analytes and MS measurement^7,8,9,10. Through immuno-enrichment, the analytes are purified from complex samples and therefore the ion-suppression effects from other molecules are minimized. Among various solid supports, magnetic beads are currently most widely used as they have the advantages of high antibody binding capacity and ease of handling. Magnetic beads with different functionalizations and sizes have been developed and commercialized for immunoprecipitation experiments. To date, immuno-enrichment on beads has been interfaced with both electrospray ionization (ESI) and MALDI-MS for protein and peptide measurement. In stable isotope standards and capture by anti-peptide antibodies (SISCAPA) technology, proteins in the samples are digested, followed by incubation with antibody-coated beads for immuno-enrichment. In "classical" SISCAPA, the captured proteotypic peptides are eluted from the beads, and measured by Liquid Chromatography-ESI-MS (LC-MS), or by direct infusion ESI-Multiple Reaction Monitoring-MS (ESI-MRM-MS)^11,12. Immuno-enrichment improved the MRM assay sensitivity by 3-4 orders of magnitude, reaching the low ng/mL range¹³.

Compared to electrospray-MS, MALDI-MS is faster, and does not involve the cleaning and re-equilibration of LC columns so there are no carryover and contamination issues, making it more suitable for high-throughput studies¹⁴. Immuno-MALDI technology has been developed in our laboratory to combine immuno-enrichment with MALDI-MS for sensitive and specific quantification of peptides and proteins (based on quantitation of proteotypic peptides)^15,16,17. After immuno-enrichment, the beads are deposited on a MALDI target plate, the matrix solution is added to beads, and the plate is ready for analysis by a MALDI-TOF-MS after drying. Elution of the peptides from the beads is not performed as a separate step, but affinity-bound analytes are eluted by the MALDI matrix solution when it is added to the bead spots, thereby simplifying the sample preparation and minimizing sample loss. The iMALDI technology has been applied in a variety of applications^18,19, and recently has been automated and used for measuring Angiotensin I (Ang I) for determining plasma renin activity (PRA)²⁰. This protocol will demonstrate the procedure used to perform an automated iMALDI assay. Taking the PRA assay as an example, inter-day CVs of less than 10% have been achieved through automation, with the capability of measuring 744 samples per day²⁰.

The iMALDI PRA assay demonstrated in this manuscript does not require protein digestion, as the target molecule (Ang I) is a peptide with a molecular weight of 1295.7 Da. In other assays where protein digestion is necessary and a peptide is used as the surrogate for the intact protein, the selected peptide for iMALDI should be unique and specific to the target protein, with a mass over 800 Da so that it can be easily distinguished from the chemical noise from the MALDI matrix solution. Anti-peptide antibodies are required for the immuno-enrichment of the peptides. The protocol for an iMALDI assay measuring PRA consists of four steps: 1) generation of Ang I in human plasma; 2) immuno-enrichment of Ang I using antibody-coated beads; 3) transfer of beads to a MALDI target plate and adding matrix solution; and 4) signal acquisition by a MALDI-TOF-MS and data analysis²⁰.

Protokół

The amounts of the reagents described below are based on the measurement of 20 patient plasma samples. The protocol presented below follows the guidelines of the University of Victoria's human research ethics committee.

1. Generation of Ang I in Human Plasma

1. Thaw plasma samples (≥200 µL) in a room temperature water bath for 5 min, and then put the samples on ice until completely thawed.
2. Transfer 200 µL of each plasma sample manually to separate wells of a 1.1 mL deep-well plate (sample plate), and centrifuge the plate in a centrifuge for 10 min at 2 °C and 1278 x g.
3. Use an automated liquid handling system to serially dilute a 500 fmol/µL Ang I NAT standard solution to prepare six calibrator solutions with chicken egg white albumin (0.1, 0.2, 0.6, 1.9, 5.7, 17.2 fmol/µL).
4. Pipette 200 µL of each calibrator to a well, and 125 µL of generation buffer to sample plate.
   NOTE: The CEWA in phosphate buffered saline (PBS) buffer needs to be freshly prepared on the day of the experiment.
5. Using an automated liquid handling system, in a new plate mix 125 µL of plasma supernatant or 125 µL of CEWA in PBS with 25 µL of Ang I generation buffer, which contains 1 M Tris, 0.2 mM ethylenediaminetetraacetic acid (EDTA), and 1 mM phenylmethylsulfonyl fluoride (PMSF).
   NOTE: Prepare the Ang I generation buffer by mixing a 1 M Tris/0.2 mM EDTA aqueous buffer (adjust to pH 5.5 using acetic acid) and a PMSF solution (100 mM in methanol). Both solutions can be stored up to 1 month at 4 °C. Mix the two solutions on the day of the experiment.
6. Automatically transfer the solutions into a 96-well plate, 3 replicates per solution, 34 µL per well. Incubate the plate at 37 °C for 3 h.

2. Immuno-enrichment of Ang I Using Antibody-coated Beads

1. Conjugation of antibody onto magnetic Protein G beads
   NOTE: Conjugation of the antibody with the beads is performed manually during the 3 h Ang I generation period on the day of the experiment. For other analytes, the conjugated beads might be able to be stored in PBS buffer containing 0.015% CHAPS (PBSC) at 4 °C for three months or longer, depending on the properties and stability of the antibody.
   1. Transfer 110 µL of bead slurry (enough for measuring 20 samples and making a 6-point standard curve) into a 1.5 mL tube. Wash the beads seven times with 1 mL of 25% acetonitrile/PBSC, and three times with 1 mL of PBSC. Use a magnetic stand to pellet the beads between each washing step. Remove the wash buffer after the last wash.
      NOTE: Washing 7 times with 25% acetonitrile/PBSC is critical for removing the MS-incompatible additives in the bead slurry, such as Tween-20. If there are no such additives in the selected beads, this extensive washing step might not be necessary.
   2. Resuspend the beads in 110 µL of PBSC, and add 110 µL of anti-Ang I antibody (final antibody concentration: 100 µg/mL). Mix the beads and solution by pipetting, and then incubate them at room temperature for 1 h, rotating at 8 rpm.
   3. Wash the beads 3 times with 1 mL of PBSC, and resuspend in 1100 µL of PBSC.
      NOTE: If any bead solution has flowed into the cap of the tube during incubation, spin down the solution using a benchtop centrifuge at 2680 x g.
   4. Transfer the bead solution manually to a 96-well plate (bead standard plate). Use the automated liquid handling system to aliquot the beads to the same 96-well plate, 120 µL per well. 
2. Immuno-enrichment on the beads
   1. After the 3 h Ang I generation period, place the incubation plate on ice for 10 min to terminate the generation of Ang I.
   2. Automatically dilute the SIS peptide stock solution (10 pmol/μL) 100-fold with PBS buffer, and further automatically aliquot the stable isoptope standard peptide dilution to a 96-well PCR plate. Transfer 1.5 µL of a stable isotope standards (SIS) peptide solution (containing 100 fmol) into each well of the incubation plate and mix it with the plasma samples or the CEWA in PBS buffers.
   3. Automatically transfer the contents of the incubation plate to the bead solution, 10 mL per well, mix.
   4. Incubate the plate at 4 °C for 1 h while rotating at 8 rpm.
   5. Wash the beads three times automatically with 5 mM ammonium bicarbonate (AmBic) solution, 100 µL per well per wash. After the last wash, resuspend the beads in 7 µL of 5 mM AmBic solution in each well. Use a magnet to pull the beads to the bottom after the last wash.

3. Transfer of Beads onto a MALDI Target Plate and Adding Matrix Solution

1. Transfer 7 µL of the bead slurry automatically onto a MALDI target plate with a spot size of 2,600 µm. Let the beads dry out.
   NOTE: A small USB-powered fan can be used to accelerate the bead drying process.
2. Automatically add 2 µL of α-cyano-4-hydroxycinnamic acid (HCCA)-matrix solution (containing 3 mg/mL HCCA, 1.8 mg/mL ammonium citrate, 70% acetonitrile, and 0.1% trifluoroacetic acid) from the matrix well onto each sample spot on the target plate.

4. Signal Acquisition by a MALDI-TOF-MS and Data Analysis

1. Analyze the sample spots with a MALDI-TOF instrument using positive reflector mode. Perform internal calibration, data smoothing, and baseline subtraction automatically with vendor-specific software.
   NOTE: The mode (positive/negative, linear/reflector) selected for MALDI-TOF measurement depends on the target peptides or proteins.
2. Calculate the relative response ratio (Nat/SIS intensity ratio) and compare it to the standard curve to determine of the Ang I concentration in each sample. Calculate PRA using Equation (1), where Δt_{Ang I generation} represents the time used for the generation of Ang I.
   PRA = [Ang I]/Δt_{Ang I generation} (1)

Wyniki

An automated iMALDI procedure for measuring Ang I is shown in Figure 1. Target peptides (either endogenous peptides or peptides from digested proteins) are enriched on anti-peptide magnetic beads, and then the beads are transferred to a target plate for MALDI measurement. The whole procedure is simplified compared to other immuno-MS technologies that require additional peptide elution steps. Automation of the iMALDI assay allows for high-throughput analysis o...

Dyskusje

Compared to conventional MS-based protein quantification, iMALDI uses antibodies to enrich the analytes and purify them from complex samples, therefore making it possible to quantify proteins or peptides at low concentrations. A critical step in the iMALDI protocol is the immuno-enrichment of the target peptides. For this purpose, antibodies with high specificity and affinity should be selected. In SISCAPA, it has been reported that antibody affinities at 10^-9 M or better would be needed to achieve high sensit...

Materiały

Name	Company	Catalog Number	Comments
Healthy control human plasma	Bioreclamation	HMPLEDTA2
Ammonium bicarbonate	Sigma Aldrich	09830
Ammonium citrate dibasic	Sigma Aldrich	09833
CHAPS (>=98%)	Sigma Aldrich	C9426
Albumin from chicken egg white (>98%)	Sigma Aldrich	A5503
Ethylenediaminetetraacetic acid	Sigma Aldrich	EDS
Alpha-cyano-4-hydroxycinnamic acid	Sigma Aldrich	70990
Phosphate buffered saline	Sigma Aldrich	P4417
Phenylmethanesulfonyl fluoride	Sigma Aldrich	78830
Trifluoroacetic acid	Thermo Fisher Scientific	85172	LC-MS grade
acetonitrile	Fluka	34967	LC-MS grade
water	Fluka	39253	LC-MS grade
acetic acid	Fluka	320099	LC-MS grade
Tris(hydroxymethyl)aminomethane	Roche Diagnostics	3118169001
Dynabeads Protein G magnetic beads	Thermo Fisher Scientific	10003D	2.8 μm, 30 mg/mL
anti-Ang I goat polyclonal antibody	Santa Cruz Biotechnology	sc-7419
Nat and SIS Ang I	synthesized at the University of Victoria-Genome BC Proteomics Centre
Automated liquid handling system	Agilent	16050-102	Agilent Bravo robotic workstation
Magnet	Thermo Fisher Scientific	12321D	Invitrogen DynaMag-2 magnet
Tube rotator	Theromo Scientific	400110Q	Labquake Tube Rotator
Magnet	Thermo Fisher Scientific	12027	DynaMag-96 side skirted magnet
Magnet	VP Scientific	771RM-1	used to pull the beads to the bottom of the well
MALDI-TOF	Bruker		Bruker Microflex LRF instrument