A Friction Testing-Bioreactor Device for Study of Synovial Joint Biomechanics, Mechanobiology, and Physical Regulation

Podsumowanie

The present protocol describes a friction testing device that applies simultaneous reciprocal sliding and normal load to two contacting biological counterfaces.

Streszczenie

In primary osteoarthritis (OA), normal 'wear and tear' associated with aging inhibits the ability of cartilage to sustain its load-bearing and lubrication functions, fostering a deleterious physical environment. The frictional interactions of articular cartilage and synovium may influence joint homeostasis through tissue level wear and cellular mechanotransduction. To study these mechanical and mechanobiological processes, a device capable of replicating the motion of the joint is described. The friction testing device controls the delivery of reciprocal translating motion and normal load to two contacting biological counterfaces. This study adopts a synovium-on-cartilage configuration, and friction coefficient measurements are presented for tests performed in a phosphate-buffered saline (PBS) or synovial fluid (SF) bath. The testing was performed for a range of contact stresses, highlighting the lubricating properties of SF under high loads. This friction testing device can be used as a biomimetic bioreactor for studying the physical regulation of living joint tissues in response to applied physiologic loading associated with diarthrodial joint articulation.

Wprowadzenie

Osteoarthritis (OA) is a debilitating, degenerative joint disease that affects more than 32 million American adults, with a healthcare and socio-economic cost of over $16.5 billion¹. The disease has classically been characterized by the degradation of articular cartilage and subchondral bone; however, changes to the synovium have recently garnered appreciation as synovitis has been linked to OA symptoms and progression^2,3,4. In primary (idiopathic) OA, normal 'wear and tear' associated with aging inhibits cartilage's ability to sustain its load-bearing and lubrication functions. The stresses generated by prolonged sliding contact of articular cartilage layers or sliding contact of cartilage against implant materials have been shown to facilitate delamination wear through subsurface fatigue failure^5,6. As a dynamic mechanical environment exists within the joint^7,8, the frictional interactions of articular cartilage and synovium may influence joint homeostasis through tissue level wear and cellular mechanotransduction. To study these mechanical and mechanobiological processes, a device has been designed to replicate the motion of the joint with tight control over compressive and frictional loading^{5,6,9,10,11,12,13}.

The present protocol describes a friction testing device that delivers reciprocal, translating motion and compressive load to contacting surfaces of living tissue explants. The computer-controlled device permits user control of the duration of each test, applied load, range of motion of the translation stage, and translation speed. The device is modular, allowing for testing of various counterfaces, such as tissue-on-tissue (cartilage-on-cartilage and synovium-on-cartilage) and tissue-on-glass. In addition to the functional measurements obtained by the tester, tissue and lubricating bath components can be assessed before and after testing to evaluate the biological changes imparted by a given experimental regimen.

Studies of cartilage tribology have been performed for decades, and several techniques have been developed to measure friction coefficients between cartilage and glass and cartilage on cartilage^14,15. The different approaches are motivated by the joint and/or the lubrication mechanism of interest. There is often a tradeoff between the control of experimental variables and the recapitulation of physiologic parameters. Pendulum-style devices utilize intact joints as the fulcrum of a simple pendulum where one joint surface translates freely over the second surface^14,16,17,18. Instead of using intact joints, friction measurements may be obtained by sliding cartilage explants over desired surfaces^{14,19,20,21,22,23,24,25}. Reported friction coefficients of articular cartilage have varied over a wide range (from 0.002 to 0.5) depending on the operating conditions^14,26. Devices have been created to replicate rotary motion^23,27,28. Gleghorn et al.²⁶ developed a multi-well custom tribometer to observe cartilage lubrication profiles using Stribeck curve analysis, and a linear oscillatory sliding motion was applied between cartilage against a flat glass counterface.

This device aims to isolate frictional responses and explore the mechanobiology of living tissues under various loading conditions. The device employs a simplified test set-up simulating joint articulation through compressive sliding, which can approximate both rolling and sliding motion with the understanding that the resistance in pure rolling motion is negligible relative to the measured friction coefficient of articular cartilage²⁹. Originally built to study the effects of interstitial fluid pressurization on the frictional response of articular cartilage⁹, the tester has since been used to explore topics such as frictional effects of removing the superficial zone of cartilage¹⁰, lubricating effects of synovial fluid¹¹, cartilage wear hypotheses^5,6,30, and synovium-on-tissue friction measurements¹³. The friction-testing bioreactor can conduct friction experiments under sterile conditions, providing a novel mechanism to explore how frictional forces affect the mechanobiological responses of living cartilage and synovium. This design can be used as a biomimetic bioreactor to study the physical regulation of living joint tissues in response to applied physiologic loading associated with diarthrodial joint articulation.

This study presents a configuration for synovium-on-cartilage friction testing over a range of contact stresses and in different lubricating baths. The articulating surface area of most joints is, to a great extent, synovial tissue³¹. While synovium-on-cartilage sliding does not occur at primary load-bearing surfaces, the frictional interactions between the two tissues may still have important implications for tissue level repair and cell mechanotransduction. It has previously been shown that fibroblast-like synoviocytes (FLS) residing on the intimal layer of the synovium are mechanosensitive, responding to fluid-induced shear stress³². It has also been demonstrated that stretch^33,34 and fluid-induced shear stress³⁵ modulate FLS lubricant production. As such, direct sliding contact between synovium and cartilage may provide another mechanical stimulus to resident cells in the synovium.

Only a few reports on synovium friction coefficients have been published^31,36. Estell et al.¹³ sought to expand on the previous characterization by utilizing biologically relevant counterfaces. With the friction testing device's ability to test living tissues, it is possible to mimic physiologic tissue interactions during joint articulation to elucidate the role of contact shear stress on synoviocyte function and its contribution to the crosstalk between synovium and cartilage. The latter has been implicated in mediating synovial joint inflammation in arthritis and post-injury. Due to the physical proximity of cartilage to synovium and synovial fluid, which contain synoviocytes that exhibit multipotent capacity, including chondrogenesis, it is postulated that synoviocytes play a role in cartilage homeostasis and repair by engrafting to the articular surface. In this context, physical contact and reciprocal shearing of cartilage-synovium and synovium-synovium may increase the accessibility of synoviocytes to regions of cartilage damage^37,38,39,40. Studies utilizing synovium-on-cartilage configurations will not only provide insights into joint gross tissue mechanics and tribology, but they may also lead to new strategies for maintaining joint health.

Protokół

Juvenile bovine knee joints, obtained from a local abattoir, were used for the present study. Studies with such bovine specimen samples are exempted from Columbia Institutional Animal Care and Use Committee (IACUC).

1. Designing the friction testing device

NOTE: A schematic representation of the friction testing device is shown in Figure 1. The device is built on a rigid base plate (not shown), which serves as a platform for structural support.

1. Attach a stepper motor to the horizontal translation stage (see Table of Materials), creating a two-axis friction testing device that delivers reciprocal translating motion to contacting surfaces.
2. Mount a multiaxial load cell on the translation stage (see Table of Materials). The mounted load cell will be used to measure the normal load in the z-direction (F_n), and the tangential load in the x-direction (F_t).
3. Equip the translation stage with a linear encoder (see Table of Materials) to record the horizontal displacement (u_x) of the stage. Further, equip the loading stage with a linear encoder (see Table of Materials) to record the vertical displacement (u_z) of the platen.
   NOTE: The translation stage encoder records the relative tangential displacement of the contacting surfaces, and this information is used to detect the beginning of each new cycle of reciprocating sliding.
4. Configure the loading platen (top contacting surface) as a glass, cartilage, or synovium counterface. Connect the platen to the loading stage via a threaded support rod.
5. Attach a two-part magnetic base to the top of the load cell (see Table of Materials): (1) a fixed base that is permanently attached to the load cell and (2) a removable base that magnetically connects to the fixed base. Ensure that the two parts form a tight connection.
   NOTE: The removable base will hold the translating counterface (bottom contacting surface).
6. Prescribe a normal load. Use dead weight mounted on linear bearings above the loading platen and support rod. Alternatively, specify a load using the voice coil actuator (see Table of Materials), which can dynamically load the bottom contacting surface⁴¹.
7. House the device within an aluminum-framed acrylic enclosure (see Table of Materials) to protect its environment from contamination.
   ​NOTE: A custom LabVIEW program controls the device (see Supplementary coding files) with user control of the duration of each test, as well as stage travel path, acceleration (change of direction), and speed. The normal force, tangential force, stage displacement, and creep displacement are monitored throughout the test with data acquisition hardware and software (see Table of Materials).

2. Specimen preparation and mounting

1. Prepare for a sterile tissue harvest following the steps below.
   NOTE: If a sterile harvest is not desired, proceed to step 2.2.
   1. Sterilize metal tools in an autoclave. Spray joint holders with 70% ethanol and place them in the biological safety cabinet (BSC). Close the cabinet for one ultraviolet (UV) cycle.
   2. Retrieve the tools from the autoclave. Place the tools, betadine, sterile scalpel blades, and beakers containing 70% ethanol in the BSC.
   3. Inside the BSC, open the tools and place them in 70% ethanol beakers. Attach the scalpel blades to scalpel handles.
   4. Prepare the joint for harvest. Spray the outside of the joint with 70% ethanol and wrap in aluminum foil for 30 min. Take care not to break the joint capsule.
      NOTE: The juvenile bovine knee joints were received with the femur and tibia cut approximately 15 cm superior and inferior to the joint to ensure an intact capsule.
   5. After 30 min, place the wrapped joint inside the BSC. Open the foil and secure the joint to its holder. Cover the joint in betadine by gently wiping the betadine across the joint surface.
      NOTE: Refer to step 2.2 and step 2.3 for synovium-specific instructions and cartilage-specific instructions, respectively.
2. Harvest the juvenile bovine synovium following the steps below.
   1. Secure the tibiofemoral joint capsule using a ring stand (see Table of Materials) with the anterior side facing the dissector. Using forceps and a scalpel blade, sever the patellar tendon using a horizontal incision of 5-10 cm (depending on joint size) superior to the tibia (Figure 2A).
   2. Hold the detached patella tendon with forceps. Make two anterior-to-posterior cuts in the shape of a V (Figure 2B,C). These cuts should free the patella.
      NOTE: As the joint begins to open, be careful not to sever the anterior cruciate ligament (ACL), posterior cruciate ligament (PCL), medial collateral ligament (MCL), lateral collateral ligament (LCL), and meniscus.
   3. Rotate the patella behind the joint or remove it completely from the joint. Carefully remove the tissue superficial to the synovial membrane on the medial and lateral sides of the joint to expose the synovium.
   4. Using a scalpel blade, trace the outline of the synovium region of interest. Using forceps, grasp one end of the synovium and gently lift to stretch the synovium distal to the underlying bone. Use a scalpel blade to remove the synovium from the bone (Figure 2D,E).
   5. Place the tissue in appropriate culture media or testing bath solution. The synovium explant may be cultured for a desired experiment or mounted and used for testing.
      NOTE: Culture media/testing bath solutions may vary based on a research group's preference. For the custom-made ones used for the present study, please see Table of Materials.
3. Harvest the juvenile bovine cartilage (femoral plugs and tibial strips).
   1. Separate the femur from the tibia by severing the ACL, PCL, MCL, and LCL. Take care not to slice the femoral condyle cartilage or slice through the meniscus to the tibial plateau. Place the separated tissues in their respective holders for dissection (step 2.3.2 for femur and step 2.3.3 for tibia).
   2. Secure the femur using a ring stand. Using a biopsy punch of desired shape and size, drive the instrument normal to the femoral condyle articular cartilage surface until reaching the bone (Figure 3A).
      1. Loosen the plug's connection to the bone by moving the punch left to right and forward to backward. Do this without removing the punch.
         NOTE: Crackling sounds may be heard as the bone separates from the cartilage.
      2. Remove the punch, and therefore the plug, from the underlying bone (Figure 3B). If necessary, repeat steps 2.3.2, 2.3.2.1, and 2.3.2.2 for the remaining untouched locations on the condyle.
         NOTE: In preparation for mounting the femoral plug onto a testing base, the deep side of the plug may need to be shaved flat. This can be done with a box cutter or scalpel.
      3. Place tissue in appropriate culture media or testing bath solution. The femoral plug may be cultured for a desired experiment or mounted and used for testing.
   3. Secure the tibia in an adjustable holder (see Table of Materials). Remove the meniscus carefully while avoiding contact with the cartilage surface (Figure 4A).
      1. On the outer edges of the tibial plateau, use a box cutter to cut perpendicular to the cartilage toward the bone. Cut completely through the cartilage to make straight edges/sides (Figure 4B). Begin the cut approximately 2 mm away from each tibial plateau edge and remove excess tissue. Score the inside edges of the cartilage (Figure 4C).
         NOTE: At this point, the bone needs to be visible underneath the cartilage on the outside edges of the tibial plateau.
      2. On the outside edges, use the box cutter to make a clean cut at the interface between the bone and cartilage (Figure 4D).
         NOTE: Cut must be parallel to the cartilage surface and approximately 5 mm inward, deep enough to start separating the cartilage and bone.
      3. To remove the tibial strip from the plateau surface, gently insert a flathead screwdriver below the cut made in step 2.3.3.2. Gently rotate the screwdriver to loosen the articular cartilage from the subchondral bone (Figure 4E).
         NOTE: Crackling sounds may be heard as the bone separates from the cartilage.
      4. As the sample loosens, slowly push the screwdriver forward until the cartilage strip detaches from the bone. Push the screwdriver toward bone, not toward cartilage. Repeat this process at multiple locations until the tibial plateau articular cartilage is completely removed from the underlying bone (Figure 4F).
      5. Using a box cutter, cut the tibial plateau surface to produce rectangular samples of desired size and thickness.
         NOTE: For the present study, 10 mm x 30 mm strips were cut, but this dimension can be varied based on desired experiment and test set-up.
      6. Place tissue in appropriate culture media or testing bath solution. The tibial strip may be cultured for a desired experiment or mounted and used for testing.
      7. If necessary, repeat steps 2.3.3.1-2.3.3.6 for the second tibial plateau.
4. Mount the synovium and the cartilage following the steps below.
   1. If desired, select a tibial strip sample to test.
      NOTE: The strip can be tested as the bottom counterface.
      1. Remove the removable magnetic base (see Table of Materials) and glue a 60 mm diameter Petri dish to the top surface of the removable base.
      2. With the Petri dish glued in place, attach the removable base to the fixed base and mark the Petri dish to indicate a sliding direction.
      3. Apply a small amount of cyanoacrylate (see Table of Materials) to the center of the dish. Align the tibial strip with the sliding direction of the stage (as indicated by the mark on the Petri dish from 2.4.1.2). Gently press the cartilage strip onto the dish. Take carenot to scratch the cartilage surface.
         NOTE: A suction tool (see Table of Materials) can apply gentle pressure to the cartilage without damaging the surface to be friction tested.
      4. Restore the removable magnetic base (with attached cartilage strip) to its paired magnetic fixed base in the friction tester. Fill the Petri dish with the desired testing bath solution. The testing bath solution must completely cover the cartilage.
   2. If desired, select a femoral cartilage plug to test.
      NOTE: The plug can be tested as the bottom or top counterface.
      1. If the femoral condyle is used as the bottom counterface, remove the removable magnetic base and glue a 60 mm diameter Petri dish to the top surface of the removable base.
         1. Apply a small amount of cyanoacrylate to the center of the dish. Gently press the cartilage plug onto the dish.
            NOTE: A suction tool can apply gentle pressure to the cartilage without damaging the surface to be friction tested.
         2. Restore the removable magnetic base (with attached cartilage plug) to its paired magnetic fixed base in the friction tester. Fill the Petri dish with the desired testing bath solution. The testing bath solution must completely cover the cartilage.
      2. If the femoral cartilage is used as the top counterface, remove the loading platen and support rod from the friction tester. If necessary, remove the existing platen and select a new platen suitable for cartilage mounting.
         1. Apply a small amount of cyanoacrylate to the platen surface. Gently press the cartilage plug onto the platen.
            NOTE: A suction tool can apply gentle pressure to the cartilage without damaging the surface to be friction tested.
         2. Restore the loading platen (with attached cartilage plug) and support rod to the friction tester. Adjust the vertical height of the loading platen such that the cartilage plug hovers over the bottom counterface and is submerged in the testing bath. Add more testing bath solution if needed.
   3. If desired, select the synovium sample to test.
      NOTE: The synovium can be tested as the bottom or top counterface.
      1. If the synovium is used as the bottom counterface, remove the removable magnetic base and glue a 60 mm diameter Petri dish to the top surface of the removable base.
         1. Glue a custom-machined circular acrylic-silicone post of the desired diameter to the center of the dish.
         2. Using forceps, place the synovium on top of the post. To secure the synovium, spread an O-ring (see Table of Materials) over its circumference.
         3. Using forceps, gently pull at the synovium to stretch tissue taught and flat beneath the O-ring. Trim excess tissue with surgical scissors.
         4. Restore the removable magnetic base (with synovium attached) to its paired magnetic fixed base in the friction tester. Fill the Petri dish with the desired testing bath solution. The testing bath solution must completely cover the synovium.
      2. If the synovium is used as the top counterface, remove the loading platen and support rod from the friction tester. If necessary, remove the existing platen and select a new circular platen suitable for synovium mounting.
         1. Using forceps, place the synovium on top of the circular platen. To secure the synovium, spread an O-ring over its circumference.
         2. Using forceps, gently pull at the synovium to stretch tissue taught and flat beneath the O-ring. Trim excess tissue with surgical scissors.
         3. Restore the loading platen (with attached synovium) and support rod to the friction tester. Adjust the vertical height of the loading platen such that the synovium hovers over the bottom counterface and is submerged in the testing bath. Add more testing bath solution if needed.

3. Friction testing

NOTE: A custom LabVIEW program and associated hardware (see Supplementary coding files) are used for these tests. Please note that the custom code was built on LabVIEW 2010 and has been maintained on this same legacy version. As a result, the code may not be forward-compatible with the most recent version of the software. The following button strikes and user interface references will only be relevant to the custom code. If working with a different software version, a similar custom program can be written by modifying the code.

1. Insert the mounted specimens (step 2.4) into the friction tester device.
   NOTE: The specimens need to be submerged in the testing bath solution but must not be in contact with one another.
2. Open the software program and prescribe test parameters: stage speed, stage acceleration, travel path (distance), and test duration (Figure 5).
   1. Open the three windows in the program: Analog Data Build MFDAQ, Initialize Load PID, and Trigger Dynamic Caller.
   2. Run the Analog Data Build MFDAQ window by pressing the Run (white arrow) button.
   3. Run the Initialize Load PID window by pressing the Run (white arrow) button.
   4. Navigate to the Stepper tab in the Trigger Dynamic Caller window. Specify the acceleration, speed, and distance of the translation stage in the user input boxes.
      NOTE: The distance value sets the wear-track half-length. In other words, the stage will move from the specified zero location (step 3.5) to the set distance value in both the positive and negative x-directions.
   5. In the Stepper tab, specify the test duration by selecting the Stepper Time Index file path. Click on the Open folder button at the bottom right of the Time-State table and select the file.
   6. Specify the test duration also in the Voice Coil tab. Navigate to the Voice Coil tab in the Trigger Dynamic Caller window. Similar to step 3.2.5, select the Voice Coil Index file path by clicking on the Open folder button at the bottom right of the Time-State table and select the file. The duration must match that of the Stepper tab.
3. Prescribe the normal load. If using dead weights, place desired weights on the linear bearings above the loading platen. Ensure that the load applied plus the weight of the loading platen and support rod do not surpass the load cell rated capacity.
4. Select the path and file name for data storage using the open folder button to the right of the Write to File? box. Save the file with a ".txt" extension.
5. Center the bottom counterface underneath the top counterface. Set this as the zero x-position.
   1. Run the Trigger Dynamic Caller window by pressing the Run (white arrow) button. In the Stepper tab, click on the Home button to move the stage to the last-saved zero x-position.
   2. If counterfaces are not aligned, move the stage by clicking on the green left and right arrow buttons. When the desired location is reached, click on the Zero button to save the current stage location as the new zero x-position. Stop the Trigger Dynamic Caller window by clicking on the Stop button.
      NOTE: The stage location can only be saved while the Trigger Dynamic Caller window is running, but the stage is not yet moving as specified by the program. Pressing the Run (white arrow) button in step 3.5.1 will initiate a 15 s timeframe before the stage begins to move. Use this 15 s timeframe to move the stage and save the desired zero location.
   3. If the desired zero x-position is not obtained on the first try, repeat step 3.5.1.
      NOTE: It may help to hit the Zero button intermittently to save the stage position as the user moves the bottom counterface underneath the top counterface. Recall that clicking the Home button will move the stage to the last position saved by the Zero button.
6. Once the top and bottom counterfaces are centered, initiate friction testing of the samples by starting the cyclic movement of the stage. To do this, run the Trigger Dynamic Caller window by pressing the Run (white arrow) button.
7. Once the stage moves, slowly bring the top counterface into contact with the bottom.
   NOTE: The applied load value can be confirmed by viewing the F_z real-time plot in the software window (Figure 5A).
8. Let the test run, collecting the friction testing data.
   NOTE: Any data recorded during step 3.5 will be overwritten. The real-time hysteresis can be viewed in the Trigger Dynamic Caller window (Figure 5C).
9. After the desired testing duration, stop the test by pressing the Stop button and unloading the specimens by raising the top counterface and moving it out of contact with the bottom counterface.

4. Data processing

NOTE: A custom MATLAB program is used for data processing (see Supplementary coding files). The code calls on the output files specified by the custom LabVIEW code.

1. Use the custom code to calculate the friction coefficient and the creep displacement (time-dependent tissue deformation) per cycle.
   1. Ensure all relevant codes are saved in the same folder: "frictioncycle_fun.m", "frictioncycle_Hysteresis_plot.m", "frictioncycle_MU_plot.m", and "frictioncycle_run.m".
      NOTE: These MATLAB codes were written to be used with the specific outputs from the aforementioned LabVIEW code. If the user has created their own code or has made modifications to the one described here, the MATLAB scripts may need to be edited to accommodate those changes.
   2. Open the frictioncycle_run.m file. Click on the Run (green arrow) button in the script. Select the raw data file to be analyzed and the desired MATLAB output save location.
      NOTE: The software may require a few minutes to process data depending on the test duration.
2. If desired, perform standard tissue assessments and media analyses on the tested explants and aliquots of the testing bath solution.

Wyniki

A synovium-on-cartilage configuration was used to friction test juvenile bovine explants. The synovium was mounted on a 10 mm diameter acrylic loading platen such that the intimal layer would be in contact with the underlying cartilage. A tibial strip was used as the cartilage counterface (Figure 6A). Tibial strips were cut with a depth of approximately 1.4 mm and a size of 10 mm x 30 mm. The samples were tested for 1 h at 37 °C in a phosphate-buffered saline (PBS) bath or a bovine syno...

Dyskusje

A dynamic mechanical environment exists within the joint as cartilage is subjected to compressive, tensile, and shear forces, and hydrostatic and osmotic pressures^44,45. Although cartilage is the main load-bearing tissue of the joint, the synovium also undergoes frictional interactions with the cartilage surface and with itself in regions where the tissue folds. The physical interactions between cartilage and synovium are likely responsible for transferring cells...

Materiały

Name	Company	Catalog Number	Comments
Aluminum foil	Reynolds Group Holdings	Reynolds Wrap	Sterile tissue harvest
Aluminum-framed acrylic enclosure	Custom made		Friction tester component
Autoclavable instant sealing sterilization pouches	Fisherbrand	01-812-54	Sterilization of tools
Autoclave	Buxton		Sterilization of tools
Beaker (250 mL)	Pyrex Vista	70000	Tissue harvest
Betadine (Povidone Iodine Prep Solution)	Medline Industries, LP	MDS093906	Sterile tissue harvest
Biological safety cabinet	Labconco	Purifier Logic+ Class II, Type A2 BSC	Sterile tissue harvest
Biospy punch	Steritool Inc.	50162	Tissue harvest
Box cutter	American Safety Razor Company	94-120-71	Tissue harvest
Circular acrylic-sillicone post (synovium)	Custom made		Tissue mounting
Culture media	Custom made		DMEM (Cat No. 11-965-118; Gibco) supplemented with 50 μg/mL L-proline (Cat. No. P5607; Sigma), 100 μg/mL sodium pyruvate (Cat. No. S8636; Sigma), 1% ITS (Cat. No. 354350; Corning), and 1% antibiotic–antimycotic (Cat. No. 15-240-062, Gibco)
Cyanoacrylate (Loctite 420 Clear)	Henkel	135455	Tissue mounting
Dead weights	OHAUS		Normal load
Ethanol 200 proof	Decon Labs, Inc.	2701	Dilute to 70 %
Fixed base	ThorLabs, Inc.	SB1T	Friction tester component
Forceps (synovium harvest)	Fine Science Tools	11019-12	Tissue harvest
Forceps (synovium mounting)	Excelta	3C-S-PI	Tissue mounting
Horizontal linear encoder (for translating stage)	RSF Electronics, Inc.	MSA 670.63	Friction tester component; system resolution of 1 µm
Hot glue gun and glue	FPC Corporation	Surebonder Pro 4000A	Tissue mounting
LabVIEW	National Instruments Corporation	LabVIEW  2010	Friction testing program
Load cell	JR3 Inc.	20E12A-M25B	Friction tester component; 0.0019 lbs resolution in x&y, 0.0038 lbs resolution in z
Loading platen	Custom made		Tissue mounting
O-ring	Parker	S1138AS568-009	Tissue mounting
Petri dish (60 mm)	Falcon	351007	Tissue mounting
PivotLok Work Positioner (tibia holder)	Industry Depot, Pivot Lok	PL325	Tissue harvest
Removable base	ThorLabs, Inc.	SB1B	Friction tester component
Ring stand			Tissue harvest
Scalpel blades	Havel's Inc.	FSC22	Tissue harvest
Scalpel handle	FEATHER Safety Razor Co., Ltd.	No. 4	Tissue harvest
Screwdriver	Wera	3334	Tissue harvest
Stage	JMAR		Friction tester component
Stepper motor	Oriental Motor Co., Ltd.	PK266-03B	Friction tester component
Suction tool	Virtual Industries, Inc.	PEN-VAC Vacuum Pen	Tissue mounting
Support rod	Custom made		Tissue mounting
Surgical scissors	Fine Science Tools	14061-09	Tissue mounting
Synovial fluid (bovine)	Animal Technologies, Inc.		Friction testing bath
Testing bath	Custom made		Phosphate-Buffered Saline (PBS) with protease inhibitors: 0.04% isothiazolone-base biocide (Proclin 950 Cat. No. 46878-U; Sigma) and 0.1% protease inhibitor - 0.05 M ethylenediaminetetraacetic acid, EDTA (Cat. No. 0369; Sigma)
Tissue culture incubator	Fisher Scientific	Isotemp	Sterile culture
Vertical linear encoder (for loading stage)	Renishaw	T1031-30A	Friction tester component; 20 nm resolution
Voice coil actuator	H2W Technologies	NCC20-15-027-1RC	Friction tester component