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Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System

17.9K Views

14:09 min

March 18th, 2010

DOI :

10.3791/1639-v

March 18th, 2010


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Genetic Studies

Rozdziały w tym wideo

0:00

Title

1:15

Introduction

3:01

Plasmid DNA Constructions

9:21

Preparing Cell Lysates for Western Blot Analysis

6:00

Genetic Analysis of Hydroxyurea (HU) or Methylmethane-sulfonate (MMS) Sensitivity in WRN Transformed Yeast Strains

7:24

Determining Cell Cycle Distribution of the WRN Transformated sgs1 top3 Strain

4:04

Streak Analysis of the Slow Growth Phenotype Restoration in WRN Transformed sgs1 top3 Strain

11:09

Effects of WRN Expression in sgs1 and top3 Yeast Cells

13:23

Conclusion

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