JoVE Logo

Zaloguj się

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

2.4K Views

06:59 min

March 31st, 2022

DOI :

10.3791/62583-v

March 31st, 2022


Przeglądaj więcej filmów

Next Generation Sequencing

Rozdziały w tym wideo

0:05

Introduction

0:41

Cell Plating

2:21

Transfection

4:42

Results: Identifying the Mutations Associated with Double Strand Break Repair near the CD4 Promoter

6:15

Conclusion

Powiązane Filmy

article

08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

13.7K Views

article

10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

7.7K Views

article

08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

9.0K Views

article

08:37

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

7.5K Views

article

07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

21.5K Views

article

09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

5.4K Views

article

07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

5.8K Views

article

08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

487 Views

article

12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

3.4K Views

article

05:01

Assessment of DNA Double Strand Break Repair Activity Using High-throughput and Quantitative Luminescence-Based Reporter Assays

1.1K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone