Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System

1:15

Introduction

3:01

Plasmid DNA Constructions

4:04

Streak Analysis of the Slow Growth Phenotype Restoration in WRN Transformed sgs1 top3 Strain

6:00

Genetic Analysis of Hydroxyurea (HU) or Methylmethane-sulfonate (MMS) Sensitivity in WRN Transformed Yeast Strains

7:24

Determining Cell Cycle Distribution of the WRN Transformated sgs1 top3 Strain

9:21

Preparing Cell Lysates for Western Blot Analysis

11:09

Effects of WRN Expression in sgs1 and top3 Yeast Cells

13:23

Conclusion