Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk Metabolomics

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03:39 min

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November 8th, 2024

DOI :

10.3791/66849-v

November 8th, 2024

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Darian T. Carroll¹, Sarah R. Lindsley², Melissa Kirigiti², Alex Buko³, Angela Jones⁴, Paul Kievit², Maureen Gannon¹^,⁵^,⁶^,⁷

¹Department of Molecular Physiology and Biophysics, Vanderbilt University, ²Division of Metabolic Health and Disease, Oregon National Primate Research Center, ³Human Metabolome Technologies (HMT) America, ⁴Vanderbilt Technologies for Advanced Genomics, Vanderbilt University Medical Center, ⁵Department of Medicine, Vanderbilt University Medical Center, ⁶Department of Veterans Affairs Tennessee Valley, ⁷Department of Cell and Developmental Biology, Vanderbilt University

Podsumowanie

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Frozen Primate Fetal Islets

Single Nuclei RNA sequencing

Embryonic Development

Postnatal Function

Metabolome Analysis

Genome Interaction

Multi omic Experiments

Batch Effects

Transcriptomic Analysis

Epigenomic Landscapes

Capillary Electrophoresis mass Spectrometry

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