Cystometry and EUS-EMG Recording Preparation in Awake Mice

To begin, implant a bladder catheter and EUS electrode in the anesthetized mouse. After waterproofing the mouse's skin, prepare it for the urodynamic procedure. Ensure that the mouse is fully recovered from anesthesia, monitoring respiratory function throughout.

Then take a 20-milliliter syringe and fill it with sterile 0.9%sodium chloride solution at room temperature. Secure the syringe to the infusion pump and set the infusion speed to 0.01 milliliters per minute. Now connect the syringe by the PE-30 tube to one side of the three-way connector.

Connect the bladder catheter to the other side and to a pressure transducer. Fix the pressure transducer at the same level as the mouse bladder. Then attach one ground line hook to the skin and the other to the electrode connector sites.

Record the pressure in the software. After starting the software, check the intravesical pressure and external urethral sphincter electromyography signals. Save the sample name and set the time.

Finally, start the pump infusion and record the signals.