To begin disease-associated gene merging, place the previously saved TXT files from five databases containing the herbal name, ingredient ID, gene ID, and the script Disease. R in the same folder. Open the R code.
Copy and paste the path where the TXT file is stored into the line where the setwd is located in the R code. Open the R software. Run the modified R code, and Save.
Set the gene and name the file Disease.txt. Open the Venn diagram website. In the Input section, copy and paste the already saved text content into the list.
Name the content with the respective database. and click Submit. Click Save Image As PNG below the image and save the text result in the same folder.
Place the alltargets.symbol. txt file and the disease. txt file in the same folder.
Open the R code. Copy and paste the path where the txt file is stored into the line where the setwd is located In the R code. Open the R software.
Run the modified R code, and Save. Set the gene and name the file Drug_Disease.txt. Open the Venn diagram website.
In the Input section, copy and paste alltargets.symbol. txt and Disease. txt into the list and name the files.
Click Save Image As PNG below the image, and save the text results in the same folder. Open the string website, and click Multiple protein. Click Browse and upload the DrugDisease.
txt file. then select Homo sapiens in Organisms. Click SEARCH, followed by CONTINUE.
Now, click Settings, and set the minimum required interaction score to the highest confidence. From the Network Display option, check Hide disconnected nodes in the network, then click UPDATE>Adjust the nodes in the network diagram so that there is no overlap or occlusion. Click Exports, followed by Download as a high resolution bitmap to get the Protein Interworking Network Map in PNG format, Download the TSV file as a short tabular text output.
Save PNG and TSV files in a unified folder.
