Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
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10:19 min
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January 10th, 2011
DOI :
January 10th, 2011
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Rozdziały w tym wideo
0:05
Title
0:45
Introduction
1:10
Injecting mRNA into Oocytes
4:26
Preparing the Ag/AgCl Wire and the Data Acquisition Equipment
1:51
Preparing the Perfusion System
5:33
Stripping the Vitelline Membrane from the Oocyte
6:14
Preparing the Patch Pipettes
7:06
Obtaining an Inside-Out Patch
8:08
Patch Clamping
8:41
Recording of Wild-Type BK Channels on and Oocyte Membrane Patch
9:57
Conclusion
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