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Recording Gap Junction Current from Xenopus Oocytes

2.1K Views

09:04 min

January 21st, 2022

DOI :

10.3791/63361-v

January 21st, 2022


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Xenopus Oocytes

Rozdziały w tym wideo

0:04

Introduction

0:42

Preparation of Defolliculated Oocytes

1:27

GJ Protein Expression

3:03

Testing the Acquisition System with the Model Cell Supplied with the Amplifier

4:09

Set Up the Recording Stage and the Reference Electrode

4:48

Set Up the VDIFF Electrodes

5:51

Set Up the Current, Voltage Electrodes, Data Acquisition

6:57

Results: Representative Recording Traces

8:05

Conclusion

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