Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

0:45

Introduction

1:10

Injecting mRNA into Oocytes

1:51

Preparing the Perfusion System

4:26

Preparing the Ag/AgCl Wire and the Data Acquisition Equipment

5:33

Stripping the Vitelline Membrane from the Oocyte

6:14

Preparing the Patch Pipettes

7:06

Obtaining an Inside-Out Patch

8:08

Patch Clamping

8:41

Recording of Wild-Type BK Channels on and Oocyte Membrane Patch

9:57

Conclusion