A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

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39.9K Views

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17:35 min

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November 1st, 2013

DOI :

10.3791/50671-v

November 1st, 2013

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Lisa M. Weatherly*¹^,², Rachel H. Kennedy*¹^,², Juyoung Shim¹, Julie A. Gosse¹^,²

¹Department of Molecular and Biomedical Sciences, University of Maine, Orono, ²Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono

Podsumowanie

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Keywords Microplate Assay

RBL 2H3 Mast Cells

Mast Cell Degranulation

Triclosan

Allergic Mediators

hexosaminidase

High throughput Screening

Organic Solvent free

UV Vis Spectrophotometry

Rozdziały w tym wideo

0:05

Title

2:45

Preparation of Cells

4:06

Preparation of Triclosan (TCS)

6:12

Antigen-stimulated Degranulation Assay Using RBL-2H3 Cells

12:13

Results: TCS Causes a Significant Dose-responsive Inhibition of Mas Cell Degranulation

16:38

Conclusion

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