JoVE Logo

Sign In

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

39.9K Views

17:35 min

November 1st, 2013

DOI :

10.3791/50671-v

November 1st, 2013


Transcript

Explore More Videos

Keywords Microplate Assay

Chapters in this video

0:05

Title

2:45

Preparation of Cells

4:06

Preparation of Triclosan (TCS)

6:12

Antigen-stimulated Degranulation Assay Using RBL-2H3 Cells

12:13

Results: TCS Causes a Significant Dose-responsive Inhibition of Mas Cell Degranulation

16:38

Conclusion

Related Videos

article

13:42

Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes

11.9K Views

article

09:20

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

12.5K Views

article

16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

13.1K Views

article

11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

12.2K Views

article

08:25

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation

2.3K Views

article

10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

3.5K Views

article

01:59

Mast Cell Degranulation Assay to Study the Effect of a Target Chemical

490 Views

article

02:30

Humanized Mediator Enzyme Release Assay: An Immunochemical Method to Screen Allergic Potential of Test Antigen by Monitoring Basophil Degranulation Response In Vitro

135 Views

article

01:29

A Degranulation Assay to Monitor Neutrophil Activation and Gelatinase Release

374 Views

article

01:30

An Assay to Monitor Degranulation of Azurophilic Granules in Neutrophils following Activation

121 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved