This study was supported by National Institutes of Health (Grant 1UM1HL112856).

All procedures and protocols were approved by the Institutional Animal Care and Use Committee of the University of Illinois at Chicago. Experiments described here are acute non-survival experiments, and there was no use of eye ointment. Maintenance of sterile conditions only occurs when surgical instruments are washed with 70% ethanol in DiH2O. Sacrifice of rats at the end of the experiment occurred via overdose of IV ketamine/xylazine.
1. Preparation of Instruments and Chemicals
<ol>
	<li>Prepare stock solution of 0.05 M of 5-HT HCl in PBS. Then dilute stock with PBS to a final 5-HT concentration of 0.203 mM.</li>
	<li>Dilute dronabinol in sesame oil at a concentration of 20 &#181;g/&#181;l.</li>
	<li>Cut 20 cm length of polyethylene (PE)-50 tubing (I.D. 0.58 mm, O.D. 0.965 mm). At one end of the tubing, cut a bevel tip using scissors, and at the other square end, insert a 23 g needle. Connect this 23 g needle to a 1 ml syringe, and fill it up with 0.3 ml of 50 u/ml heparin.</li>
	<li>Cut four pieces of 4-0 braided silk thread and set to the side.</li>
	<li>Sterilize surgical scissors, 2 curved Graefe forceps, and 3 micro clamps with 70% ethanol in diH2O.</li>
</ol>
2. Catheterization of Femoral Vein
Protocol for catheterization is modified from Jespersen et al.21
<ol>
	<li>Anesthetize a Sprague-Dawley rat with intraperitoneal (IP) ketamine/xylazine (100 mg/kg:10 mg/kg). Pinch the toe of the rat and observe any movement to confirm a proper level of anesthesia. Shave the ventral aspect of the left thigh, and the ventral aspect of the neck. If necessary by the local Institutional Animal Care and Use Committee, apply eye ointment to prevent dryness.</li>
	<li>Secure rat in a supine position on a surgical board. Use surgical scissors to cut away skin in the left hind thigh.</li>
	<li>Using forceps, conduct blunt dissection of the superficial muscle to expose the femoral vein. Use the forceps to separate the vein from the femoral artery, and place 2 threads around the femoral vein.</li>
	<li>Use the curved Graefe forceps to pull-up the vein to stop blood flow. Using a 22 G syringe needle, puncture the femoral vein and then insert the beveled end of the PE-50 tubing into the vein.</li>
	<li>Check if the PE-50 tubing was inserted correctly by retracting the plunger of the syringe. Blood should be seen entering the PE-50 tubing. Tie 2 knots around the vein and PE-50 tubing using the 2 threads.</li>
</ol>
3. 5-HT-induced Apnea via IV Infusion
<ol>
	<li>Place a piezoelectric strain gauge around the rat to measure respiration.</li>
	<li>Change settings for amplification level of the electronic amplifier via amplifier software on the computer: to amplify (100x) and band-pass filter (1-10 Hz) the respiratory signals obtained from the strain gauge, set &#8220;highpas filter&#8221; at &#8220;AC @ 1 HZ&#8221; and &#8220;Lowpass Filter&#8221; at &#8220;10 Hz,&#8221; and set the amplification by inputting &#8220;10&#8221; for the &#8220;Initial Gain&#8221; and &#8220;100&#8221; for &#8220;Total Gain.&#8221;.</li>
	<li>Change settings for sampling rate by opening &#8220;Analog Input&#8221; via recording software on the computer. To digitize (500 Hz sampling rate) using an analog-to-digital converter, &#8220;sample&#8221; at &#8220;1,000 Hz&#8221; and &#8220;skip&#8221; every other recording point by &#8220;1&#8221; (effective sampling rate 500 Hz), and then record the signal using recording software.</li>
	<li>Remove the 1 ml syringe from the catheter and insert a 500 &#181;l precision glass syringe filled with 0.203 mM of 5-HT into the catheter.</li>
	<li>Place the 500 &#181;l precision glass syringe into an infusion pump. Infuse 12.5 &#181;g/kg per 350 &#181;l/kg of 5-HT solution at a rate of 63 ml/hr. Perform multiple infusions and observe apnea in rat, which is seen as a pause in breathing (&#8805; 2.5 sec) in the recorded respiratory signals on the computer monitor.</li>
	<li>Before proceeding with neck surgery, monitor breathing pattern and check for pain reflex from toe pinch in the rat. If breathing is irregular, or if there is a pain reflex from toe pinch, administer IP ketamine/xylazine (100 mg/kg:5 mg/kg) and then re-confirm a proper level of anesthesia.</li>
</ol>
4. Neck Surgery to Expose Nodose Ganglia
<ol>
	<li>Secure rat in a supine position on a surgical board. Make a midline longitudinal cut using surgical scissors at the neck.</li>
	<li>Using blunt dissection on the platysma muscle (using 2 micro clamps to keep this muscle clear of the surgical site), expose the sternohyoideus and omohyoideus muscles. Separate these muscles to expose the internal carotid artery and one of its branches, the pterygopalatine artery.</li>
	<li>Observe the vagus nerve as it runs along the internal carotid artery and then along the pterygopalatine artery. Observe how the vagus nerve and pterygopalatine artery, along with the glossopharyngeal nerve (cranial nerve IX) and the spinal accessory nerve (cranial nerve XI), enter the posterior lacerated foramen at the base of the skull.</li>
	<li>Notice that the nodose ganglion is displayed as a swelling of the vagus nerve right before it enters the posterior lacerated foramen. Also notice that the pharyngeal and laryngeal nerve branches come off the anterior and posterior aspects, respectively, of the nodose ganglion.5</li>
	<li>Using the Graefe forceps, separate the vagus nerve from the arteries, and place a piece of thread around the vagus nerve. Place a clamp on the thread to apply slight tension on the vagus nerve, and clean the nodose ganglion of any connective tissue to provide less resistance when injecting.</li>
	<li>Repeat 5-HT-induced apnea as stated in section 3 to confirm that no damage was done to the nodose ganglia.</li>
</ol>
5. Intranodose Ganglia Injection of Dronabinol
<ol>
	<li>Fill a 10 &#181;l gastight precision glass syringe affixed with a custom-made 28 g half-inch syringe needle with a 35&#176; beveled tip with 5 &#181;l of dronabinol in sesame oil.</li>
	<li>Place a micro clamp on the thread to apply slight tension on the vagus nerve, and puncture the nodose ganglion taking care not to puncture through it. Depress the syringe slowly (&#8805; 60 sec) to inject the entire contents of the syringe. Note that some of the syringe contents will leak out of the ganglion.</li>
	<li>Repeat 5-HT-induced apnea as stated in section 3.</li>
</ol>

<ol>
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H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beyond+acid+suppression:+new+pharmacologic+approaches+for+treatment+of+GERD.">Beyond acid suppression: new pharmacologic approaches for treatment of GERD.</a> Curr. Gastroenterol. Rep. 12 (3), 175-180 (2010).</li><li>Maher, S. A., Dubuis, E. D., Belvisi, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=G-protein+coupled+receptors+regulating+cough.">G-protein coupled receptors regulating cough.</a> Curr. Opin. Pharmacol. 11 (3), 248-253 (2011).</li><li>Greene, E. 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Physiol. 304 (11), 1017-1023 (2011).</li><li>Kollarik, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transgene+expression+and+effective+gene+silencing+in+vagal+afferent+neurons+in+vivo+using+recombinant+adeno-associated+virus+vectors.">Transgene expression and effective gene silencing in vagal afferent neurons in vivo using recombinant adeno-associated virus vectors.</a> J. Physiol. 588 (21), 4303-4315 (2010).</li><li>Zhang, Z., Zhang, C., Zhou, M., Xu, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Activation+of+opioid+mu-receptors,+but+not+delta-+or+kappa-receptors,+switches+pulmonary+C-fiber-mediated+rapid+shallow+breathing+into+an+apnea+in+anesthetized+rats).">Activation of opioid mu-receptors, but not delta- or kappa-receptors, switches pulmonary C-fiber-mediated rapid shallow breathing into an apnea in anesthetized rats).</a> Respir. Physiol. Neurobiol. 183 (3), 211-217 (2012).</li><li>Calik, M. W., Radulovacki, M., Carley, D. 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Ther. 260 (2), 917-924 (1992).</li><li>Kopczynska, B., Szereda-Przestaszewska, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=5HT2+and+5HT3+receptors'+contribution+to+modeling+of+post-serotonin+respiratory+pattern+in+cats.">5HT2 and 5HT3 receptors' contribution to modeling of post-serotonin respiratory pattern in cats.</a> Life Sci. 75 (19), 2281-2290 (2004).</li><li>Fan, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cannabinoid+agonists+inhibit+the+activation+of+5-HT3+receptors+in+rat+nodose+ganglion+neurons.">Cannabinoid agonists inhibit the activation of 5-HT3 receptors in rat nodose ganglion neurons.</a> J. Neurophysiol. 73 (2), 907-910 (1995).</li><li>Jespersen, B., Knupp, L., Northcott, C. 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The authors declare that they have no competing financial interests.

The critical steps for successful injection of neurochemicals into the nodose ganglia are: 1) identifying and cleaning the connective tissue off the nodose ganglia; 2) confirming the integrity of the nodose ganglia before injection; 3) and using a small gauge needle to delicately inject into, but not completely puncturing through, the nodose ganglia.
The vagus nerve innervates many organs in the neck and abdomen, and relays important information such as heart rate, blood pressure, bronchopulmo...

Afferent signaling via the vagus nerve (cranial nerve X) transmits important general visceral information to the central nervous system (CNS) from baro-, chemo-, hepatic osmo-, cardiac, pulmonary, and gastric receptors located in the organs of the abdomen and thorax. The vagus nerve communicates information from stimuli such as heart rate, blood pressure, bronchopulmonary irritation, and gastrointestinal distension to the nucleus of solitary tract (NTS) of the medulla. The cell bodies of the pseudounipolar neurons of the vagus nerve are located in the nodose and petrosal ganglia, of which the majority is found in the former. Nodose ganglion cells contain a wealth of receptors for amino acids, monoamines, neuropeptides, and other neurochemicals that when activated, can modify afferent vagus nerve activity.1 Numerous innervations of the afferent vagus nerves coupled with the diversity of receptors located on the nodose ganglia illustrate the biological importance of this cranial nerve, and systemic drugs that do not cross into the CNS targeted at receptors on nodose ganglia can be used to treat various diseases, such as sleep apnea, gastroesophageal reflux disease, or chronic cough.2-4
The ease of access to the nodose ganglia lends itself to experimental manipulation by midline longitudinal incision made at the neck. The vagus nerve emerges from the posterior lacerated foramen at the base of the skull, and immediately displays a swelling of the nerve that is the nodose ganglion. The nodose ganglion is easily recognizable due to two nerve branches that arise from it: anteriorly the pharyngeal branch; and posteriorly superior laryngeal branch.5 Previous experimental manipulations of the nodose ganglia involved electrophysiological recordings,6 injections of immunohistochemical or immunofluorescent compounds for nerve tracings,7-10 superfusion or injections of neuroexcitotoxins,11-13 injections of adeno-associated virus to knockdown receptors,14,15 and injections of receptor-specific neurochemicals to change the activity of the vagus nerve.16,17
Systemic injections of neurochemicals are problematic in that systemic treatment affects both peripheral and central nervous systems. Thus, systemic treatment does not isolate the effect of neurochemicals on afferent vagal nerve activity. This protocol describes a method using readily available equipment of intranodose injections in the Sprague-Dawley rat that modulates vagus nerve activity without affecting the central nervous system. Stimulation of serotonin type 3 (5-HT3) receptors on nodose ganglia by intravenous (IV) infusion of serotonin (5-HT) induces the Bezold-Jarisch reflex, a vagal response trifecta of bradycardia, hypotension, and apnea, which can be abolished by supranodose vagatomy.11,17-19 Apnea is easily measured by placing a respiratory transducer around the abdomen of the rat.17,18 Cannabinoids decrease 5-HT-induced current in nodose ganglia cells,20 and intranodose ganglia injections of dronabinol attenuate 5-HT-induced apnea.

<table border="0" cellpadding="0" cellspacing="0" width="660">
	<tbody>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Name of Material/ Equipment</td>
			<td style="width:155px;">Company</td>
			<td style="width:141px;">Catalog Number</td>
			<td style="width:175px;">Comments/Description</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">5-HT HCl</td>
			<td style="width:155px;">MP Biomedicals</td>
			<td style="width:141px;">215376591</td>
			<td style="width:175px;">12.5 &#181;g/kg per 350 &#181;l/kg</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Dronabinol (Marinol) 10 mg Capsules (80 &#181;g/&#181;l)</td>
			<td style="width:155px;">AbbVie</td>
			<td style="width:141px;">NDC 0051-0023-21</td>
			<td style="width:175px;">Dilute with sesame oil to 20 &#181;g/&#181;l</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Sesame Oil</td>
			<td style="width:155px;">Sigma-Aldrich</td>
			<td style="width:141px;">S3547</td>
			<td style="width:175px;"></td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Intramedic Polyethylene-50</td>
			<td style="width:155px;">BD</td>
			<td style="width:141px;">427411</td>
			<td style="width:175px;">Ordered from VWR (Cat. # 63019-047)</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Graefe Forceps</td>
			<td style="width:155px;">Roboz</td>
			<td style="width:141px;">RS-5138</td>
			<td style="width:175px;">Two are needed</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Johns Hopkins Bulldog Clamp</td>
			<td style="width:155px;">Roboz</td>
			<td style="width:141px;">RS-7441</td>
			<td style="width:175px;">Three are needed</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Piezoelectric Strain Gauge</td>
			<td style="width:155px;">Ambu</td>
			<td style="width:141px;">813255-100</td>
			<td style="width:175px;"></td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Data Acquisition USB Subsystems</td>
			<td rowspan="2" style="width:155px;">DataWave Technologies</td>
			<td style="width:141px;">NA</td>
			<td style="width:175px;"></td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Sciworks Experimenter Software</td>
			<td style="width:141px;">NA</td>
			<td style="width:175px;"></td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">CyberAmp</td>
			<td style="width:155px;">Axon Instruments</td>
			<td style="width:141px;">NA</td>
			<td style="width:175px;"></td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Syringe, 500 &#181;L, Model 1750 TLL</td>
			<td rowspan="3" style="width:155px;">Hamilton Company</td>
			<td style="width:141px;">81220</td>
			<td style="width:175px;"></td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Syringe, 10 &#181;L, Model 1801 RN</td>
			<td style="width:141px;">7659-01</td>
			<td style="width:175px;"></td>
		</tr>
		<tr height="53">
			<td height="53" style="height:53px;width:191px;">Needle, 28 gauge, Small Hub RN</td>
			<td style="width:141px;">7803-02</td>
			<td style="width:175px;">Point Style 4, Angle 35, Length 0.5 in</td>
		</tr>
	</tbody>
</table>

a method of nodose ganglia injection in sprague-dawley rat

Afferent signaling via the vagus nerve transmits important general visceral information to the central nervous system from many diverse receptors located in the organs of the abdomen and thorax. The vagus nerve communicates information from stimuli such as heart rate, blood pressure, bronchopulmonary irritation, and gastrointestinal distension to the nucleus of solitary tract of the medulla. The cell bodies of the vagus nerve are located in the nodose and petrosal ganglia, of which the majority are located in the former. The nodose ganglia contain a wealth of receptors for amino acids, monoamines, neuropeptides, and other neurochemicals that can modify afferent vagus nerve activity. Modifying vagal afferents through systemic peripheral drug treatments targeted at the receptors on nodose ganglia has the potential of treating diseases such as sleep apnea, gastroesophageal reflux disease, or chronic cough. The protocol here describes a method of injection neurochemicals directly into the nodose ganglion. Injecting neurochemicals directly into the nodose ganglia allows study of effects solely on cell bodies that modulate afferent nerve activity, and prevents the complication of involving the central nervous system as seen in systemic neurochemical treatment. Using readily available and inexpensive equipment, intranodose ganglia injections are easily done in anesthetized Sprague-Dawley rats.

Figure 1 represents sample breathing recording in rats that had infusion of 5-HT to induce apnea before and after intranodose ganglia injections of dronabinol. 5-HT activates 5-HT3 receptors on the nodose ganglia that contribute to the Bezold-Jarisch reflex of bradycardia, hypotension, and apnea.11,17-19 Intranodose ganglia injections of dronabinol activate inhibitory CB receptors, or allosterically modulates 5-HT3 receptors that inhibit the 5-HT-induced excitation of the...

Watch this Scientific Journal Video about A Method of Nodose Ganglia Injection in Sprague-Dawley Rat at JoVE.com

A Method of Nodose Ganglia Injection in Sprague-Dawley Rat

Afferent vagal signaling transmits important information to central nervous system from receptors located in organs of the abdomen and thorax. The nodose ganglia of vagus nerves contain many types of receptors that modulate vagal activity. This protocol describes a method of local injections of neurochemicals into the nodose ganglia.

Afferent signaling via the vagus nerve transmits important general visceral information to the central nervous system from many diverse receptors located ...

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JoVE Journal

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18.5K Views.  University of Illinois at Chicago. Afferent vagal signaling transmits important information to central nervous system from receptors located in organs of the abdomen and thorax. The nodose ganglia of vagus nerves contain many types of receptors that modulate vagal activity. This protocol describes a method of local injections of neurochemicals into the nodose ganglia.