Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77

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7.4K Views

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12:30 min

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August 17th, 2015

DOI :

10.3791/52963-v

August 17th, 2015

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Pierre Leblanc¹, Minho Moon¹^,², Woori Kim¹, Inhye Jeong¹, Chun-Hyung Kim¹, Kwang-Soo Kim¹

¹Molecular Neurobiology Laboratory, Psychiatry, McLean Hospital, ²Department of Biochemistry, College of Medicine, Konyang University

Podsumowanie

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Polyclonal Antibodies

Cross reactivity

DNA Binding Domain

Ligand binding Domain

Affinity Chromatography

Protein A

Pre adsorption

Rozdziały w tym wideo

0:05

Title

1:31

Coupling of LBD Proteins to AminoLink Coupling Resin

4:11

Blocking Remaining Sites

5:43

Purification of Nurr1 Specific Antibodies

7:21

ELISA-based Analysis of Purified Anti-Nurr1 Antibody

10:04

Results: Pre-adsorption Against Cross-reactive Antigens Increases Specificity of Anti-Nurr1 Polyclonal Antibodies

11:56

Conclusion

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