Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77

7.4K Views

12:30 min

August 17th, 2015

DOI :

10.3791/52963-v

August 17th, 2015


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Nurr1

Chapters in this video

0:05

Title

1:31

Coupling of LBD Proteins to AminoLink Coupling Resin

4:11

Blocking Remaining Sites

5:43

Purification of Nurr1 Specific Antibodies

7:21

ELISA-based Analysis of Purified Anti-Nurr1 Antibody

10:04

Results: Pre-adsorption Against Cross-reactive Antigens Increases Specificity of Anti-Nurr1 Polyclonal Antibodies

11:56

Conclusion

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