Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma

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14.7K Views

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09:17 min

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January 15th, 2016

DOI :

10.3791/53287-v

January 15th, 2016

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Aaron J. Clark¹, Shayan Fakurnejad², Quanhong Ma², Rintaro Hashizume²^,³

¹Department of Neurological Surgery, University of California San Francisco, ²Department of Neurological Surgery, Northwestern University Feinberg School of Medicine, ³Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine

Podsumowanie

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Keywords Bioluminescence Imaging

Immunocompetent Animal Model

Glioblastoma

GL261 Murine Tumor Cells

Luciferase Modification

Tumor Cell Proliferation

In Vivo Monitoring

Tumor Growth

Immunotherapy

In Vitro Proliferation Assay

MTS Reagent

Absorbance Measurement

Rozdziały w tym wideo

0:05

Title

1:00

Induction of GL261 Firefly Luciferase Reporter Expression

2:31

In Vitro Proliferation Assay

3:40

Tumor Cell Implantation

6:16

Bioluminescence Imaging of GL261 Tumor Growth

7:26

Results: Representative In Vitro and In Vivo GL261 Luciferase Expression

8:31

Conclusion

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