Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing

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14.8K Views

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16:08 min

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January 20th, 2018

DOI :

10.3791/56275-v

January 20th, 2018

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Ahmed Elaswad*¹^,², Karim Khalil*¹^,³, David Cline¹, Patrick Page-McCaw⁴, Wenbiao Chen⁴, Maximilian Michel⁵, Roger Cone⁵, Rex Dunham¹

¹School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, ²Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, ³Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, ⁴Department of Molecular Physiology and Biophysics, Vanderbilt University, ⁵Life Science Institute, University of Michigan

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Rozdziały w tym wideo

0:00

Title

1:14

Brood Stock Selection and Spawning

4:16

Sperm Preparation

5:33

Egg Collection and Fertilization

8:07

Needle Pulling and Loading

10:20

Microinjection of Catfish Embryos

12:34

Results

15:24

Conclusion

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