Microinjection of CRISPR/Cas9 protein into channel catfish, Ictalurus punctatus embryos for gene editing. The development of a reliable and efficient protocol for targeted gene editing in channel catfish, is crucial to studying functional genomics
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A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.