Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

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7.2K Views

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11:15 min

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March 2nd, 2018

DOI :

10.3791/56398-v

March 2nd, 2018

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Vid Leko¹^,², Smitha Sripathy¹, Robin L. Adrianse¹, Taylor Loe¹, Angela Park¹, Uyen Lao¹, Eric J. Foss¹, Marisa S. Bartolomei³, Antonio Bedalov¹^,⁴

¹Clinical Research Division, Fred Hutchinson Cancer Research Center, ²Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, ³Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, ⁴Departments of Medicine and Biochemistry, University of Washington

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Keywords Pooled ShRNA Screen

MeCP2

Inactive X Chromosome

Mouse Tendon Fibroblasts

Rozdziały w tym wideo

0:04

Title

1:17

Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression

4:31

Screening the Library for Reactivation Using Hygromycin B Selection

6:44

Validating the Identified Hairpins ( Hits )

9:27

Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance

10:17

Conclusion

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