Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype
3.1K Views
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14:51 min
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June 17th, 2022
DOI :
June 17th, 2022
•Rozdziały w tym wideo
0:00
Introduction
0:24
Selection of the Most Potent Promoter to Obtain Persistent and Prolonged Expression of the shRNAs
2:01
Preparation of Pooled Lentiviral Human Epigenetic Factor shRNA Library
4:48
Estimation of the Transduction Efficiency of Lentiviruses
6:03
Transduction of Pooled Epigenetic shRNA Library in the Drug-Resistant Cell Line
7:20
Enrichment of GFP Positive Cells
7:54
Dropout Screening to Identify Epigenetic Factors Mediating Drug Resistance
9:20
Amplification of the Integrated shRNAs by PCR
11:08
Next-Generation Sequencing and Data Analysis
11:57
Representative Results
14:30
Conclusion
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