An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

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26.9K Views

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07:44 min

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May 22nd, 2020

DOI :

10.3791/61054-v

May 22nd, 2020

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Hannes Steinkellner¹, Alexander V. Beribisky¹, Philip Mausberg¹, John Christodoulou², Barbara Scheiber-Mojdehkar³, Anna Huber¹, Victoria Sarne¹, Franco Laccone¹

¹Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), ²Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, ³Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

Podsumowanie

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Electrochemiluminescence

Protein Stock Solution

Lysate Samples

HDF Lysates

Rozdziały w tym wideo

0:04

Introduction

0:35

Preparing Solutions and Plates (Day 1)

1:50

Blocking and Sample Addition (Day 2)

3:42

Antibody Addition

5:17

Reading the Plate

6:10

Results: Accuracy and Consistency of MeCP2-ECLIA

7:20

Conclusion

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