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Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

9.2K Views

19:23 min

January 16th, 2019

DOI :

10.3791/58824-v

January 16th, 2019


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Recombinant Fusion Proteins

Rozdziały w tym wideo

0:00

Title

1:05

Generation of the Substrate-coding Expression Plasmids

2:16

Expression of the Fluorescent Substrates

3:35

Cell Disruption

4:26

Ni-NTA Magnetic Agarose Bead-based Protease Assay

12:39

PAGE Analysis

14:41

Representative Results

18:29

Conclusion

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