Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
11.2K Views
•
11:32 min
•
February 22nd, 2019
DOI :
February 22nd, 2019
•Rozdziały w tym wideo
0:04
Title
1:02
Isolation of Short Posterior Ciliary Arteries
2:17
Sample Preparation
3:43
Pellet Digestion
4:40
Sample Cleaning and Buffer Exchange
5:40
One-dimensional Gel Electrophoresis (1DE)
6:50
In-gel Tryptic Digestion
9:23
Results: Comparison of Protein Extraction Efficacy and Robustness and Representative 1DE Gel Analysis
10:51
Conclusion
Powiązane Filmy
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
14.0K Views
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
27.5K Views
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
26.8K Views
Sample Preparation for Mass Cytometry Analysis
16.0K Views
An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis
13.5K Views
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
38.4K Views
Miniaturized Sample Preparation for Transmission Electron Microscopy
19.4K Views
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
5.0K Views
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
2.5K Views
Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
3.7K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone