A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene

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7.8K Views

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08:22 min

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September 16th, 2019

DOI :

10.3791/59963-v

September 16th, 2019

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Huilin Wang*¹, Xiaofan Zhu*²^,³, Baoheng Gui*³^,⁴, Wan Chee Cheung², Mengmeng Shi²^,³, Zhenjun Yang²^,³, Ka Yin Kwok², Ricky Lim⁵, Sanna Pietilä⁵, Yuanfang Zhu⁶^,⁷, Kwong Wai Choy²^,³

¹Central Laboratory, Bao'an Maternity and Child Health Hospital, Jinan University, ²Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, ³Shenzhen Research Institute, The Chinese University of Hong Kong, ⁴Department of Genetics and Metabolism, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, ⁵PerkinElmer Diagnostics, ⁶Department of Obstetrics and Gynecology, Bao'an Maternity and Child Health Hospital, Jinan University, ⁷Maternal-Fetal Medicine Institute, Bao'an Maternity and Child Health Hospital, Jinan University

Podsumowanie

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Polymerase Chain Reaction

PCR Assay

Fragile X Syndrome

Trinucleotide Repeats

Molecular Diagnosis

Genetic Screening

FXS Disorders

DNA Sample Preparation

PCR Master Mix

Thermocycler Protocol

Rozdziały w tym wideo

0:04

Title

0:44

PCR Amplification

2:20

Purification of the PCR Products

3:52

Fragment Sizing of PCR Products

5:32

-SB"

6:18

Results: Quantification of CGG Repeats of FMR1

7:29

Conclusion

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