A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene
7.8K Views
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08:22 min
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September 16th, 2019
DOI :
September 16th, 2019
•Huilin Wang*1, Xiaofan Zhu*2,3, Baoheng Gui*3,4, Wan Chee Cheung2, Mengmeng Shi2,3, Zhenjun Yang2,3, Ka Yin Kwok2, Ricky Lim5, Sanna Pietilä5, Yuanfang Zhu6,7, Kwong Wai Choy2,3
1Central Laboratory, Bao'an Maternity and Child Health Hospital, Jinan University, 2Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, 3Shenzhen Research Institute, The Chinese University of Hong Kong, 4Department of Genetics and Metabolism, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, 5PerkinElmer Diagnostics, 6Department of Obstetrics and Gynecology, Bao'an Maternity and Child Health Hospital, Jinan University, 7Maternal-Fetal Medicine Institute, Bao'an Maternity and Child Health Hospital, Jinan University
Chapters in this video
0:04
Title
0:44
PCR Amplification
2:20
Purification of the PCR Products
3:52
Fragment Sizing of PCR Products
5:32
-SB"
6:18
Results: Quantification of CGG Repeats of FMR1
7:29
Conclusion
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