Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

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7.1K Views

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10:03 min

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November 5th, 2019

DOI :

10.3791/60047-v

November 5th, 2019

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Chiara Pagliuca¹, Elena Scaglione¹, Francesca Carraturo¹, Giuseppe Mantova¹, Maria Michela Marino¹, Maria Virginia Pishbin¹, Caterina Pagliarulo², Roberta Colicchio¹, Paola Salvatore¹^,³

¹Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, ²Department of Science and Technology, Sannio University, ³CEINGE – Advanced Biotechnology

Podsumowanie

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Meningococcal Meningitis

Neisseria Meningitidis

Intracisternal Delivery

Murine Model

Host pathogen Interaction

Passive Immunotherapy

Bacterial Inoculation

GC Broth

Optical Density

Experimental Protocol

Pathogens

Disease Induction

Therapeutic Strategy

Rozdziały w tym wideo

0:05

Title

1:48

Preparation of Bacteria for In Vivo Infection Studies

3:09

Intra-cisternal Injection of Mice

5:07

Evaluation of Colony Forming Units (CFU) Counts in Peripheral Organs

6:39

Preparation of Brain Tissues for CFU Count

8:05

Results: Survival of Mice Following Intracisternal Injection of Wild-type and Mutant N. meningitidis

9:28

Conclusion

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