Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
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5.5K Views
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06:39 min
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August 19th, 2020
DOI :
August 19th, 2020
•Jennifer Eymael1, Laura Miesen1, Fieke Mooren1, Jitske Jansen1,2, Jack Wetzels3, Johan van der Vlag4, Bart Smeets1
1Radboud Institute for Molecular Life Sciences, Department of Pathology, Radboud University Medical Center, 2Radboud Institute for Molecular Life Sciences, Amalia Children's Hospital, Department of Paediatric Nephrology, Radboud University Medical Center, 3Radboud Institute for Health Sciences, Department of Nephrology, Radboud University Medical Center, 4Radboud Institute for Molecular Life Sciences, Department of Nephrology, Radboud University Medical Center
Rozdziały w tym wideo
0:04
Introduction
0:39
Mouse Kidney Dissection
1:04
Isolation of Glomeruli from Mouse Kidney
2:25
Culturing of Glomerular Outgrowth
3:40
Analysis of Parietal Epithelial Cell Proliferation\
4:47
Results: Analysis of Glomerular Outgrowth
6:08
Conclusion
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