Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis

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5.7K Views

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11:51 min

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November 18th, 2019

DOI :

10.3791/60435-v

November 18th, 2019

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Xianquan Zhan¹^,²^,³^,⁴, Huanni Li⁵, Shehua Qian¹^,²^,³, Xiaohan Zhan¹^,²^,³, Na Li¹^,²^,³

¹Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, ²Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, ³State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, ⁴National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, ⁵Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University

Podsumowanie

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Mitochondria Preparation

Ovarian Cancer Tissues

Control Tissues

Quantitative Proteomics

Differential speed Centrifugation

Density Gradient Centrifugation

ITRAQ Proteomics

Mitochondrial Isolation Buffer

Tissue Homogenization

Proteome Changes

Biomarkers

Therapeutic Targets

Rozdziały w tym wideo

0:04

Title

1:49

Preparation of Mitochondria from Human Ovarian Cancer Tissues

5:00

Preparation of Mitochondria from Human Control Ovarian Tissues

8:44

Results: Analysis of the Prepared Mitochondrial Samples

10:42

Conclusion

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