Preparation of Mitochondria from Human Ovarian Cancer Tissues
5:00
Preparation of Mitochondria from Human Control Ovarian Tissues
8:44
Results: Analysis of the Prepared Mitochondrial Samples
10:42
Conclusion
Transkript
Mitochondrial changes play important roles in human ovarian cancers. This protocol establish an effective platform to isolate and purify mitochondria from human ovarian cancer and the control tissues for large-scale proteomics analysis. Mitochondr
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This article presents a protocol of differential-speed centrifugation in combination with density gradient centrifugation to separate mitochondria from human ovarian cancer tissues and control ovarian tissues for quantitative proteomics analysis, resulting in a high-quality mitochondrial sample and high-throughput and high-reproducibility quantitative proteomics analysis of a human ovarian cancer mitochondrial proteome.