Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
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4.9K Views
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13:53 min
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December 19th, 2020
DOI :
December 19th, 2020
•Rozdziały w tym wideo
0:00
Introduction
0:54
Synthesis of the UAA: Propargyl-L-Lysine (Prk)
4:46
Production of the Recombinant Protein modified by PrK
8:13
Removal of the Histidine Tag by TEV Protease Digestion
9:48
Assessment of PrK Accessibility and Functionality and Conjugation to the Hapten
11:26
Results: Efficient Incorporation of the PrK and Click Conjugation
12:49
Conclusion
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