Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

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14.2K Views

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10:05 min

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May 23rd, 2020

DOI :

10.3791/60822-v

May 23rd, 2020

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Rita Soares¹^,²^,³, Filipa F. Ribeiro¹^,², Diogo M. Lourenço¹^,², Rui S. Rodrigues¹^,², João B. Moreira¹^,², Ana M. Sebastião¹^,², Vanessa A. Morais¹^,³, Sara Xapelli¹^,²

¹Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, ²Instituto de Farmacologia e Neurociências, Faculdade de Medicina, Universidade de Lisboa, ³Instituto de Biologia Molecular, Faculdade de Medicina, Universidade de Lisboa

Podsumowanie

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Rozdziały w tym wideo

0:04

Introduction

0:59

Postnatal Day 1-3 (P1-3) Mouse Brain Harvest

2:28

Subventricular Zone (SVZ)/Dentate Gyrus (DG) Microdissections

4:22

Tissue Dissociation

5:27

Postnatal Neural Stem Cell Expansion

6:09

Neurosphere Passaging

7:10

Results: Representative Neurosphere Expansion and Characterization

9:21

Conclusion

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